N-乙烯基吡咯烷酮接枝改性聚乳酸组织工程支架的制备与性能

Preparation and properties of N-vinyl pyrrolidone grafted PLA engineering scaffolds

摘要:

利用热致相分离技术制备了N-乙烯基吡咯烷酮接枝改性聚乳酸（M-PLA）组织工程支架。在36%接枝率下，讨论了聚合物浓度、水/二氧六环比例和粗化温度对支架孔隙度和微孔结构的影响；并进一步探讨了在相同制备工艺条件下，不同接枝率对支架结构的影响，并对M-PLA支架的亲水性和蛋白粘附性能进行测试。结果显示，在一定接枝率下，支架孔隙度随聚合物浓度增大而降低；水的添加有利于规则孔径的形成；粗化温度降低，支架孔径和孔隙度提高。与PLA支架相比，随着接枝率提高，共聚物支架孔隙度变化不大，孔径略减小，但孔隙规整性和连通性较好，亲水性和蛋白粘附率明显增大，生物相容性提高。

Abstract:

N-vinyl pyrrolidone grafted polylactic acid (M-PLA) scaffold for tissue engineering was fabricated by thermal induced phase separation technique. The effect of polymer concentration, water/ dioxane ratio and coarsening temperature on the scaffold porosity and structure were studied with grafting ratio of 36%. And the effect of grafting ratio on the scaffold structure was also studied with the same preparation condition. The hydrophilisity and protein adhesion of M-PLA scaffold was tested. The results showed that, the porosity of the M-PLA scaffold with grafting ratio of 36% decreased with the increase of polymer concentration, water was helpful to form a regular porous structure, the pore size and the porosity of the scaffold increased with the decrease of coarsening temperature. With increasing grafting ratio, there were no significant changes of the porosity and pore size of the scaffold, but better pore regularity and connectivity compared with PLA scaffold. The hydrophilisity and protein adhesion test showed that the M-PLA scaffold had higher hydration, protein adhesion ratio and better biocompatibility.