双对羟基苯甲醛Schiff碱的合成及与DNA作用

Synthesis and Interaction with DNA of Bis(p-hydroxybenzaldehyde) Schiff Base

摘要:

以1,4-二（3-氨基丙基）哌嗪(BAPP)与对羟基苯甲醛（PHBA）为原料，合成了含哌嗪环的双对羟基苯甲醛Schiff碱,N,N'-二(4-羟基苄基)-1,4-二(3-亚氨基丙基)哌嗪；产物结构经元素分析、红外光谱、核磁共振氢谱和紫外光谱进行确认。采用紫外吸收光谱法和黏度法研究了标题化合物与小牛胸腺DNA (CT-DNA)的相互作用，发现紫外吸收光谱出现了减色效应和红移现象，DNA的相对粘度随着化合物浓度的增大而增加，这些现象说明该双Schiff碱以插入方式与CT-DNA结合

Abstract:

Bis(p-hydroxybenzaldehyde) Schiff base containing piperazine ring，N,N'-bis(4- hydroxybenzyl)-1,4-bis(3-iminopropyl)piperazine, was synthesized with 1,4-bis(3-amino- propyl)piperazine (BAPP) and p-hydroxybenzaldehyde (PHBA) as raw materials. The structure of the product was characterized by means of elemental analysis, IR, 1HNMR and UV-vis spectra. The interaction of the title compound with CT-DNA was investigated by UV-vis absorption spectroscopy and viscosimetry. The hypochromic effect and red shift were observed in the UV-vis absorption spectra, and the relative viscosity of CT-DNA increased with the addition of the title compound. The results indicated that the bis-Schiff base binds to CT-DNA by intercalation mode.