酶法制备猪肺抗氧化肽

Preparation of Antioxidant Peptides by Enzymatic Hydrolysis from Porcine Lung

摘要:

为了分离制备猪肺抗氧化肽，该文研究了中心组合设计和响应面分析优化猪肺抗氧化肽的提取工艺，以DPPH自由基清除率为响应值，分析了料液比、水解时间和酶浓度对制备抗氧化肽的影响，再通过葡聚糖凝胶Sephadex G-50、G-25和G-10对抗氧化肽进行分离纯化，得到了具有抗氧化活性的肽段，测定其清除DPPH自由基、超氧阴离子、ABTS自由基、羟基自由基以及金属螯合的能力。猪肺抗氧化肽的最佳提取工艺参数为：新鲜猪肺质量与水质量的比例为（料液比）1:3，水解6h，酶浓度为6500U/g，此时DPPH自由基清除率为66.89%；抗氧化活性最强的组分A5清除DPPH自由基、超氧阴离子、ABTS自由基和羟基自由基的IC50分别为0.073g/L、0.989 g/L、0.192 g/L和1.261 g/L，金属螯合能力的IC50为6.505 g/L；其相对分子质量为175.00Da。

Abstract:

In order to separate and prepare the antioxidant peptide, this article used central composite design and response surface to optimize the extraction process of antioxidant peptide.Taking DPPH radical scavenging rate as response, analyzed the influence of solid-liquid ratio, hydrolysis time and enzyme concentration on the preparation of antioxidant peptides. Got the peptide with antioxidant activity after Sephadex G-50、G-25and G-10 used to separate and purify it, and then measured the DPPH radical scavenging, Superoxide anion radical scavenging activity, ABTS radical scavenging, hydroxyl radical scavenging activity and metal ion chelating activity. The optimum extraction conditions as follows: Solid-liquid ratio was 1:3, extraction time 6h and enzyme concentration 6500U/g at the same time the DPPH radical scavenging was 66.89%. The IC50 of the DPPH radical scavenging, Superoxide anion radical scavenging activity, ABTS radical scavenging, hydroxyl radical scavenging activity were 0.073g/L、0.989 g/L、0.192 g/L and 1.261 g/L, respectively. The IC50 of metal ion chelating activity was 6.505 g/L, the molecular weight was 175.00 Da.