温敏性双亲水含半乳糖共聚物的合成及其胶束的自组装

Synthesis and self-assemble of Thermosensitive Double-hydrophic Galactose Polymer by RAFT Polymerization

摘要:

采用酶促法合成与可逆加成-链断裂转移自由基聚合(RAFT聚合)有效结合的方法，制备分子量分散系数(PDI)较低的温敏性含糖共聚物Poly(DEGMA-co-OVNG)。通过核磁共振氢谱(1H NMR)，傅里叶变换红外光谱(FT-IR)，凝胶渗透色谱(GPC)对聚合物结构进行了确认表征。通过紫外-可见光谱(UV-vis)研究表明，共聚物的低临界溶解温度(LCST)可以通过共聚单体的比例进行调控，当DEGMA与OVNG的物质的量之比为8:1时，含糖共聚物的LCST值为36 oC。在37 oC生理温度下，Poly(DEGMA-co-OVNG)可以自组装形成纳米胶束，透射电子显微镜(TEM)显示自组装形成的聚合物胶束是结构均匀、形貌规整的球形，通过动态光散射(DLS)测得纳米微球的粒径约为121 nm，该温敏性含糖共聚物在药物载体方面具有潜在的应用前景。

Abstract:

Poly(DEGMA-co-OVNG) with narrow polydispersity (PDI) and obvious thermosensitivity was effectively prepared via enzymatic synthesis combined with Reversible Addition-Fragmentation Chain Transfer (RAFT) polymerization. The structure and molecular weight of the synthesized glycopolymer were characterized by 1H NMR, FTIR and gel permeation chromatography (GPC), respectively. Measurement by Dynamic Light Scattering (DLS) and transmission electron microscopy (TEM) revealed that Poly(DEGMA-co-OVNG) was able to self-assemble into nano-micelles with well-defined structure and uniform size in aqueous solution. UV-visible spectroscopy tests showed that the low critical solution temperature (LSCT) of the polymer can be controlled by the molar ratio of monomers. Especially, as the molar ratio of DEGMA and OVNG was 8:1, the LCST of Poly(DEGMA-co-OVNG) could be 36oC in aqueous solution. The results demonstrate that Poly(DEGMA-co-OVNG) can form nano-micelles in the human body environments, which may have potential as functional drug carriers.