栝楼籽蛋白质提取工艺优化及抗氧化性

Optimization of Ultrasonic-Assisted Extraction and Antioxidant Activity of Protein from seed of Trichosanthes kirilowii Maxim

摘要:

以栝楼籽为原料，采用超声波辅助碱法提取栝楼籽蛋白质。通过单因素实验方法和正交实验方法确定了最佳提取工艺条件；考察所得栝楼籽蛋白质对DPPH自由基和羟基自由基的清除能力。结果表明：栝楼籽中超声波辅助碱法提取栝楼籽蛋白质的最佳提取工艺为：料液比为1:30（g:mL），提取液为pH 10含氯化钠的浓度0.7 mol/L的碱液，在70 ℃条件下用200 W超声辅助提取30 min，栝楼籽中蛋白质提取率可达到22%左右。当栝楼籽蛋白质溶液浓度为0.8 mg/mL时，对DPPH自由基的清除率为66.46%，对羟基自由基的清除率为49.23%，其清除能力随浓度的增大而增强；其清除能力强于柠檬酸而弱于抗坏血酸。

Abstract:

Choose lye as extraction solvent, protein from seed of Trichosanthes kirilowii Maxim was extracted by ultrasonic technology. The optimal ultrasonic-assisted extraction technology was determined by single factor experiment and orthogonalexperiment. Meanwhile，antioxidant activity of protein t on DPPH free radical and hydroxyl radical was evaluated. Results showed: the optimal ultrasonic-assisted extraction technology of protein in seed of Trichosanthes kirilowii Maxim was: lye as extraction solvent of pH 10 including 0.7 mol/L NaCl, material-to-liquid ratio of 1:30 (g:mL), ultrasonic power of 200 W , ultrasonic-assisted extracting time about 30 min and extraction temperature at 70℃. The extraction rate of protein reached about 22% with the optimal ultrasonic-assisted extraction technology. When the protein concentrationt was 0.8 mg/mL, the scavening rate on DPPH free radical and the scavening rate on hydroxyl radical were 66.46%, 49.23% respectively.The scavenge capability of DPPH free radical and hydroxyl radical increased along with the increase of the protein concentrationt, it was superior to citric acid but inferior to ascorbic acid.