双水相萃取黄连生物碱的研究

Study on extraction of rhizoma coptidis alkaloids using aqueous two-phase system

摘要:

利用聚乙二醇(PEG)和硫酸铵（(NH4)2SO4）形成的双水相体系萃取黄连生物碱。通过双水相体系的改变，分别对黄连中4种主要生物碱：小檗碱、黄连碱、巴马丁、药根碱在双水相中的分配行为进行研究,并结合紫外分光光度法检测筛选出黄连生物碱最佳萃取工艺。最佳萃取工艺参数：pH = 6，T = 20 ℃，PEG1000质量分数20%，(NH4)2SO4质量分数16%，小檗碱、黄连碱、巴马丁、药根碱的最高萃取率分别为99.79%,，98.04%，99.96%，99.39%。双水相萃取黄连生物碱高效环保可行。

Abstract:

Extraction of rhizoma coptidis alkaloids using aqueous two-phase system of polyethylene glycol (PEG) and ammonium sulfate ((NH4)2SO4). Through the change of the aqueous two-phase system, researched the distribution behavior of four major alkaloids in rhizoma coptidis: berberine, coptisine, palmatine and jatrorrhizin in aqueous two-phase system, and combined with ultraviolet spectrophotometry to detect out the best extraction craft of rhizoma coptidis alkaloids. The best extraction process parameters: pH = 6, T = 20 ℃, PEG1000 mass fraction 20%, ((NH4)2SO4) mass fraction 16%, the highest extraction of berberine, coptisine, palmatine and jatrorrhizin are 99.79%, 98.04%, 99.96%,99.39%, respectively. Aqueous two-phase extraction of rhizoma coptidis alkaloid efficient environmental protection is feasible.