有机溶剂对介孔材料固定化α-葡萄糖苷酶活性影响

Immobilization of α-Glucosidase on a Mesoporous Material in Different Organic Solvents

摘要:

以聚乙二醇-聚丙二醇-聚乙二醇三嵌段共聚物（P123）为模板剂，1，3，5-三甲基苯（TMB）为扩孔剂，用1,2-双-三乙氧基硅基乙烷（BTESE）和(3-氰丙基)三乙氧基硅烷（CTES）合成介孔材料，并将α-葡萄糖苷酶固载其中，研究常见有机溶剂对该固定化酶活性的影响。结果表明，体积分数分别为25%、25%、25%、25%和10%的5种有机溶剂25% 乙醇、25%甲醇、25%异丙醇、25%丙酮和10%乙腈50 h内日间酶活性保持情况 分别稳定在内日间剩余酶活性分别稳定在90%、45%、40%、25%和42%，可见有机溶剂会对固定化酶的酶活性产生一定影响，但仍保留部分酶活性。

Abstract:

PolyoxyethylenePolyoxyethylene-polyoxypropylene-polyoxyethylene (P123) was used as a template and 1,3,5-trimethylbenzene (TMB) was used as a swelling agent. A mesoporous material was synthesized using 1,2-bis(triethoxysilyl)ethane(hexaethoxydisilethylene) (BETS) and (3-cyanopropyl)triethoxysilane (CTES), and α-glucosidase was successfully immobilized on this material. The effects of 5 organic solvents on the immobilized enzyme were then investigated. Over 50 h, the following residualstable residual enzyme activities were observed: 90% in 25% ethanol, 45% in 25% methanol, 40% in 25% isopropanol, 25% in 25% acetone and 42% in 10% acetonitrile. Although there are certain limitations for α-glucosidase immobilized on mesoporous materials in organic solvents, some activity remained. Therefore, mesoporous materials are expected to be utilized to immobilize α-glucosidase in subsequent research and industrial applications.