丙醇/硫酸铵双水相体系分离纯化茶皂素的研究

Study on separation and purification of tea saponin with propyl alcohol/ammonium sulphate aqueous two-phase system

摘要:

以茶饼中提取的茶皂素为粗品原料（茶皂素质量分数为40.2%），采用丙醇/硫酸铵双水相体系对粗品茶皂素进行分离提纯。考察了硫酸铵质量分数、丙醇质量分数、萃取温度、萃取pH对茶皂素产品萃取率和产品含量的影响。结果表明：该工艺分离速度快，产品萃取率和含量较高，在硫酸铵质量分数为20%、丙醇质量分数为23%、温度为40 ℃、pH为7时，茶皂素萃取率可达到89.32%，茶皂素质量分数可达到78.12%。

Abstract:

With the crude saponin(40.2%) extracted from tea cake as raw material.An aqueous two-phase system based on propyl alcohol/ammonium sulfate was used in separation and purification of saponin.The influences of such factors as the concentration of ammonium sulfate,the concentration of propyl alcoho,temperature and pH were investigated.The results show that, the process separate very speed,product extration ratio and content is high.They were listed as follow:ammonium sulfate concentration 20%,propyl alcoho concentration 23%,temperature 40℃ and pH 7 adjustment.Under the optimal conditions,the extration ratio and the content reached 89.32% and 78.12%.