人参皂苷Re分子印迹整体柱的制备与吸附研究

Study of Adsorption and Preparation of Ginsenoside Re Molecularly Imprinted Monolithic Column

摘要:

以人参皂苷Re作为模板分子，以甲基丙烯酸为功能单体，二甲基丙烯酸乙二醇酯为交联剂，异丙醇和十二醇为致孔剂，偶氮二异丁腈为引发剂，原位热引发聚合制备人参皂苷Re分子印迹整体柱，并考察整体柱对Re的分离与吸附性能。结果表明：实验最适流动相为甲醇：水=40:60(v/v)，整体柱的分离因子为1.769，采用迎头法测定吸附等温线表明吸附遵循反langmiur型等温线模型，饱和吸附量为0.247mg/g，整体柱表现出对Re的特异性吸附性能。扫描电镜表明聚合物内部形成独特孔隙结构，利于流动相的流动和模板分子的传质。

Abstract:

The molecularly imprinted monolithic column(MIMC) was synthesized using α-methacrylic acid as functional monomers, ethylene glycol dimethacrylate as cross-linker, the mixture of isopropanol and dodecadol as porogenic reagent and Ginsenoside Re as template molecules by in-situ polymerization. The performances of separation and adsorption of MIMC were investigated, the results of research indicated that optimal mobile phase was methanol and water (40:60, v/v), separation factor 1.769, the adsorption isotherm followed the anti-langmiur isotherm model by frontal chromatography, saturated adsorption capacity was 0.247mg/g. MIMC has special molecule recognition ability to Re. The result of scanning electron microscopy(SEM) showed that the unique porous structure was formed in the polymer, which was beneficial to the flow of mobile phase and the mass transfer of template molecules.