聚酰胺树脂分离纯化箬叶总黄酮的工艺研究

Study on purification technology of total flavonoids from Indocalamus leaves with polyamide resin

摘要:

采用单因素和正交试验，优化了聚酰胺树脂分离纯化箬叶总黄酮的工艺条件，并进行了梯度洗脱。结果表明，最佳上样条件为：上样液浓度2.60 mg/mL，上样量20 mL，上样流速1.0 mL/min；最佳洗脱条件为： 体积分数为80%的乙醇，洗脱剂用量150 mL，洗脱流速0.5 mL/min，此条件下平均解吸率为94.54%，其中洗脱剂用量及其浓度为显著因素。梯度洗脱物的显色反应可鉴定出黄酮类、黄酮醇类及异黄酮类。

Abstract:

By using single factor and orthogonal experiments, the optimum conditions in the purification of total flavonoids from Indocalamus leaves with polyamide resin were studied. The results showed that the optimal sample condition was 2.60 mg•mL-1 in sample concentration, 20 mL of sample volume, sample flow rate of 1.0 mL•min-1, and the optimum elution condition was eluent of 80%（V/V） ethanol, elution liquid amount of 150 mL, the elution rate of 0.5 mL•min-1, the average desorption rate could run up to 94.54%，the amount and concentration of the eluent were significant factors. flavonoids, flavonols and isoflavones could be identified in color reaction of gradient elution.