超声醇提柿木皮单宁的工艺优化及抗油脂氧化研究

Optimization of Ultrasonic-assisted Extraction of Tannin from Persimmon Veneer and Their Antioxidation Activity

摘要:

选用超声醇提柿木皮中的单宁。通过单因素和正交试验优化提取工艺；用过氧化值（POV）和丙二醛（MDA）吸光度值分析方法考察了柿木皮单宁粗提物和纯化物的油脂抗氧化活性，并探讨了粗提物和纯化物分别与维生素C（VC）的协同作用。结果表明：柿木皮单宁的最佳提取工艺为超声波功率360 W、温度70 癈、乙醇浓度50%、提取时间50 min，该条件下单宁得率为5.6 %；粗提物和纯化物都对油脂有一定的抗氧化活性，它们的抗氧化化活性大小顺序为：纯化物>粗提物，即柿木皮单宁纯度越高抗氧化性能越强。当粗提物添加量为0.08 %时，有较强抗油脂氧化活性，与VC没有物协同增效作用；0.04 %纯化物有很好抗氧化作用，其抗氧化效果优于VC但和VC组合无协同增效作用。

Abstract:

Using ethanol as extraction solvent, tannin was extracted under ultrasonic assistance from persimmon veneer. The optimal ultrasonic-assisted extraction technology was determined by single factor experiment and orthogonal experiment; The antioxidant activity of crude extract and purification were determined by POV measured values and the absorbance values of MDA. The results show that The optimum values of extraction parameters including ethanol ratio, extraction power, extraction temperature and length of extraction were determined by orthogonal array design to be: 50%, 360 W, 70 癈,50 min and the tannin yield was 5.6%. Both the crude extract and purification have antioxidant activity, with an antioxidant sequence of purification > crude extract. Especially, the higher purity had better antioxidant activity . The crude extract exhibits antioxidation for oil when the dosage is 0.08 %, but and it hasn’t synergistic action with VC. 0.04 % purification had good antioxidant effect which is better than VC, but it hasn’t synergistic action with VC.