辐射交联PVA/PVP/胶原复合水凝胶的制备及性能研究

Preparation and Characterization of PVA/PVP/collagen Composite Hydrogel by Irradiation Crosslinking

摘要:

通过一步辐射交联与冻融循环相结合的方法，将胶原引入PVA水凝胶体系，形成交联网络结构，制备具有较高生物活性的PVA/PVP/胶原复合水凝胶。通过含水率、溶胀性能及力学性能表征PVP含量及胶原的添加对复合水凝胶性能的影响，并优选最佳体系进行生物相容性测试。结果表明，通过辐射交联与冻融循环相结合的方法，可以获得具有较高溶胀性能的含胶原水凝胶支架；胶原的加入有利于提高水凝胶的含水率和溶胀性能，但水凝胶力学性能降低。生物相容性研究表明，与PVA/PVP水凝胶支架相比，含胶原复合水凝胶生物相容性大大提高，更有利于促进细胞生长。

Abstract:

PVA/PVP/ collagen composite hydrogel with high biological activity was prepared by a combination of irradiation crosslinking and freezing/ thawing cycles. The effect of PVP content and collagen on water content, swelling property and mechanical property of the hydrogel were studied. The biocompatibility of PVA/PVP and PVA/PVP/collagen hydrogel was tested by MTT. The results showed that PVA/PVP/collagen hydrogel scaffolds with high swelling property can be obtained by the combination of radiation crosslinking and freezing/ thawing cycles. Collagen was helpful to improve the swelling performance of PVA/PVP hydrogel while the mechanical property was decreased. Biocompatibility study showed that PVA/PVP/collagen hydrogel had better biocompatibility compared with the PVA/PVP hydrogel, and was more conducive to promote cell growth.