水溶性吲哚菁染料的合成、光谱性能及其应用的研究

Research on Synthesis and Spectral Properties of Water-Soluble Indocyanine Dye and Its Application

摘要:

设计、合成了3种水溶性吲哚菁染料（发射波长：690 nm），用核磁共振（NMR）对其进行了表征，测试了菁染料在不同溶剂中的紫外吸收光谱和荧光发射光谱；为了探讨菁染料在生物分析领域的应用性，测定3种菁染料在十六烷基三甲基溴化铵（CTAB）和十二烷基磺酸钠（SDS）两种表面活性剂胶束模拟的生理条件下的吸收和发射光谱，利用循环伏安法（CV）测定了菁染料的光稳定性，在近红外区域观察了染料对肾癌细胞的活细胞染色性能，并计算了3种菁染料的摩尔吸光系数和荧光量子产率。

Abstract:

3 novel water-soluble indocyanine dyes (em. 690 nm) were designed, synthesized and characterized by nuclear magnetic resonance (NMR) for fluorescent labeling of biomolecules. Spectral properties of the prepared dyes in different solvents were tested. In order to investigate the application of fluorescent dyes in the field of biomedical analysis, the absorption and emission spectra of the dyes in the surfactants as CTAB, SDS were measured, the photostability of dyes were measured by cyclic voltammetry (CV). The application of the new dyes in cellular imaging has been demonstrated through direct conjugating of the dyes with living renal carcinoma cells, then imaging was visualized by near-infrared fluorescence microscopy. The molar extinction coefficient and the fluorescence quantum yield of the dyes were calculated.