响应面法优化闪式提取葫芦巴多糖及其保润性能研究

Response Surface Methodology for Optimization of Flash Extraction for Fenugreek(Trigonella foenum-graecum L．) Polysaccharides and Research of Its Humectant Properties

摘要:

利用闪式提取方法，以葫芦巴多糖(FPS)提取率为响应值，首先通过单因素实验考察液料比、提取时间、温度、提取电压和提取次数的影响，初步优化提取条件；进而通过Box-Behnken实验设计和响应面分析法综合优化最终提取条件；并对葫芦巴多糖进行卷烟物理和感官保润性能测试。结果表明葫芦巴多糖的最佳提取条件为：液料比 27:1(mL/g)，闪式提取时间136 s，提取温度58 ℃，电压140 V ；在最优条件下进行了5次验证实验，葫芦巴多糖的平均提取率为21.23%，与理论值（21.80%）的相对误差为2.61%，响应面优化闪式提取葫芦巴多糖的方法可行；葫芦巴多糖作为保润剂在相对湿度较低的环境下，对烟丝的保湿能力强于丙二醇样品，在湿度较高的环境下，葫芦巴多糖具有防潮效果；且能够改善卷烟吸食品质，葫芦巴多糖适宜的添加量为烟丝质量的0.005%～0.1%。

Abstract:

Single factor experiment was applied to invest the effects of ratio of raw materials, extraction time, temperature, motor voltage and times on extraction yield of fenugreek polysaccharides(FPS) by using the flash extract technology. Preliminary extraction conditions were obtained. Box-Behnken design and response surface methodology were used to optimize the ultimate extraction conditions. Cigarette physical and sensory humectant properties of FPS were tested. The results indicated that the optimum conditions were as follows: the ratio of liquid to raw materialswas 27:1(mL/g), the extraction time was 136 s, the extraction temperature was 58 ℃, and the motor voltage was 140 V. Yield of FPS was 21.23% under the optimal extraction conditions and the relative error was 2.61% compared with the theoretical value (21.80%), which showed that method for FPS was feasible. Cut tobacco moisture ability with FPS was stronger than with the samples of propylene glycol under the environment of low relative humidity. FPS had moisture-proof effect at a moderately high humidity. FPS could improve the quality of cigarette smoking, and adding 0.005%～0.1% of cut tobacco weight, FPS showed the most appropriate flavoring effect.