红外热成像法研究半胱氨酸修饰金纳米棒的光热抗肿瘤效果

NIR sensitive hyperthermia antitumor of cysteine modified gold nanorods via Infrared thermal imaging method

摘要:

通过配体交换法制备出两性离子半胱氨酸修饰的金纳米棒 (Cys-AuNRs)以用于临床光热疗法。并在200mW∙cm-2的近红外激光辐照下，对Cys-AuNRs进行了光热效果测试、红外热成像分析、细胞活性和荧光成像实验。当Cys-AuNRs的浓度为57μg /mL，Hela细胞的存活率,仍高达90.1%，而光照10min后，细胞存活率则锐减到60.1%。研究结果表明Cys-AuNRs具有良好的生物相容性和较低的细胞毒性，且在近红外照射下，能够显著地杀死肿瘤细胞。因此，用生物相容性好的半胱氨酸修饰金纳米棒，在无创伤高热治疗肿瘤的临床应用上具有重要的现实意义

Abstract:

Cys-AuNRs were prepared via ligands exchange method using zwitterionic cysteine to modify AuNRs for the clinical photothermal therapy. And the photothermal effect, the infrared thermal imaging, hyperthermia effect on tumor cells and fluorescence imaging experiment of Cys-AuNRs were investigated under irradiation by an 808 nm NIR laser at a power density of 200mW∙cm-2. The survival rate of HeLa cells incubated with Cys-AuNRs at the concentration of 57 μg/mL, still remains 90.1%. However, under NIR irradiation for10 min , the survival rate of Hela cells sharply decreases to 60.1%. The results suggest that Cys-AuNRs have good biocompatibility and low cytotoxicity, can also kill tumor cells effectively under the laser radiation. Therefore, the modification of AuNRs with biocompatible cysteine has an important practical significance in the clinical noninvasive hyperthermia treatment.