多功能氟硼吡咯聚乙二醇锰肿瘤纳米成像剂的制备及性能研究

Synthesis, Properties and the Tumor Image of Multifunctional Borondipyrromethene Polyethylene Glycol Manganese Nanoparticles

摘要:

该文首先以端基为对甲苯磺酸酯活化的甲氧基聚乙二醇（PEG-OTs）为原料，与氨基乙酸甲酯发生亲核取代及水解反应生成具有氨基和羧基的双官能团聚合物（PEG-NHCH2COOH）；其次，PEG-NHCH2COOH通过C-N共价键与疏水性荧光染料氟硼吡咯结合形成新型的荧光氟硼吡咯聚乙二醇氨基酸衍生物（B-BODIPY）；再次，B-BODIPY与金属二价锰盐配位后,运用微乳液自组装方法获得多功能氟硼吡咯聚乙醇锰肿瘤纳米成像剂 (G2MnNPs)。然后，通过红外光谱（FTIR）、 UV-vis和TEM表征了合成的G2MnNPs纳米粒子。细胞成像和线粒体成像实验显示该复合物具有线粒体靶向性、肿瘤荧光成像和磁共振成像的特性，是一种低毒性多功能肿瘤成像剂。

Abstract:

In this article, a bifunctional polymer (PEG-NHCH2COOH) with amino group and carboxyl group was synthesized by nucleophilic substitution of methoxy polyethylene glycol tosylate (PEG-OTs) and methyl N-acetylglycinate, and hydrolysis reaction. Next, the hydrophobic fluorescent dye borondipyrromethene (BODIPY) was conjugated with the bifunctional polymer (PEG-NHCH2COOH) by C-N covalent bonds resulting a new fluorescent polyethylene glycol amino acid derivative (B-BODIPY). After, B-BODIPY was coordinated with the Mn(II) ion, multifunctional borondipyrromethene polyethylene glycol manganese(II) nano-particles (G2MnNPs) was formed through microemulsion self-assembly method. The nano-particles (G2MnNPs) were characterized by infrared spectroscopy (FT-IR), UV-vis and TEM testing methods. Cell imaging and mitochondria imaging experiments showed that the G2MnNPs was a low toxic multifunctional tumor imaging agent including mitochondrial targeting, fluorescence imaging and magnetic resonance imaging in cancer cells.