潜在的T2类造影剂Fe3O4@BSA纳米粒子的合成及性能研究

Synthesis and Property of Fe3O4@BSA Nanoparticles as Promising T2 Contrast Agents

摘要:

该文成功地制备出Fe3O4@BSA磁性纳米功能材料，包裹在Fe3O4纳米粒子表面的牛血清蛋白（BSA）主要起到分散剂和稳定剂的作用。通过傅里叶变换红外光谱(FTIR)、X射线衍射(XRD)、透射电子显微镜(TEM)及热重分析(TGA)对合成的Fe3O4@BSA纳米粒子进行表征和分析。结果表明，Fe3O4@BSA纳米粒子被成功地制备出来，其中BSA质量分数约为18.9 %。体外成像结果表明，随着Fe3O4@BSA纳米粒子浓度的增加，T2成像信号增强，具有明显的阴性造影效果。于0.5 T外磁场下，测得Fe3O4@BSA纳米粒子的横向弛豫率 (transverse relaxivity，r2)为148.18 L/(mmol·s)。结果表明，Fe3O4@BSA纳米粒子能够作为一种潜在的T2类造影剂。

Abstract:

Fe3O4@BSA nanoparticles (NPs) were successfully synthesized, and bovine serum albumin (BSA) coating upon the surface of Fe3O4 NPs played the role of stabilizer and disperser. The prepared Fe3O4@BSA NPs were characterized and analyzed by Fourier transform infrared spectra (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM) and thermogravimetric analysis (TGA). The results suggested the successful synthesis of Fe3O4@BSA NPs. The content of BSA was about 18.9 % in Fe3O4@BSA NPs. The results of MR imaging in vivo showed that the T2 MRI signals were improved gradually with increased iron concentrations, which presented an obvious negative MRI effect of Fe3O4@BSA NPs. The transverse relaxivity (r2) of the Fe3O4@BSA NPs was evaluated as 148.18 L/(mmol·s) at 0.5 T. These preliminary studies indicated that the obtained Fe3O4@BSA NPs could be used as promising T2 contrast agents for magnetic resonance imaging.