大孔树脂富集多舌飞蓬总咖啡酸酯的工艺研究

Study on the operating parameters of total caffeoylquinic acid ester of Erigeron multiradiatus with macroporous resins

摘要:

摘要：考察了13种不同极性的大孔树脂对多舌飞蓬中总咖啡酸酯的富集纯化能力，发现极性树脂NKA-2的静态吸附率为92.51%、解吸率为98.51%，明显优于其他树脂。进一步以NKA-2极性树脂对多舌飞蓬总咖啡酸酯进行静态吸附与动态吸附实验，结果表明，最佳上样质量浓度为300 g/L，最佳上样流速为2 BV/h，树脂吸附容量为6 mL/g，即单位NKA-2树脂可以处理1.8 g生药。解吸实验结果表明，体积分数为60 %的乙醇溶液更有利于多舌飞蓬总咖啡酸酯的洗脱，用量为8 BV。用上述条件对多舌飞蓬总咖啡酸酯富集纯化，使总咖啡酸酯的纯度由18.09 %提高到58.06 %，回收率高达94.78 %。说明NKA-2型大孔吸附树脂综合性能良好，可用于多舌飞蓬中总咖啡酸酯成分的富集和纯化。

Abstract:

Abstract: Thirteen microporous resins with different polarity were evaluated for the adsorption properties of the total caffeoylquinic acid ester(TCaf) from Erigeron multiradiatus. Macroporous resin NKA-2 with strong polarity displayed the best adsorption capacity among the tested resins. The static adsorption and dynamic adsorption test of NKA-2 for TCaf from Erigeron multiradiatus were investigated.The results show that the optimal purification parameters were sample concentration of 300 g/L,the loaded amount of 6 mL/g,and loaded flow rate of 2 BV/h. By analyzing the TCaf from Erigeron multiradiatus washed out from the resin,it was shown that the desorption effect of 60% ethanol of 8 BV was the best.The purity of TCaf increased to 58.06% after the purification,and the yield was 94.78%.NKA-2 is an ideal resin with the best enrichment efficiency for the TCaf of Erigeron multiradiatus.