基于B16黑素瘤细胞评价体系的竹叶黄酮纳米粒子美白功效研究

Study on Whitening Efficacyt of Bamboo Leaf Flavonoids Nanoparticles Based on B16 Melanoma Cell Evaluation System

摘要:

以竹叶黄酮为载药、芫根酸性多糖为包材，以ε-聚赖氨酸为阳离子桥，采用离子交联法获得竹叶黄酮纳米粒子（BLFN），平均粒径在400 nm左右，结构稳定，分散性良好。以业界公认的美白因子熊果苷为阳性对照，研究BLFN对B16小鼠黑素瘤细胞株体外培养过程中美白相关指标的影响。结果显示：BLFN对B16细胞内酪氨酸酶活性、黑色素合成和细胞增殖均表现出浓度依赖的抑制作用，当试样浓度100 μg/mL时，其抑制率分别达到69.68%、62.35%和59.14%，均显著优于熊果苷（p<0.05）。B16细胞的体外试验结果表明，BLFN能通过多种途径起到美白功效，显示出替代熊果苷的潜力。同时，由于该纳米粒子的构建成分均为天然物，具有较高的使用安全性，在美白类产品中的应用前景十分广阔。

Abstract:

The bamboo leaf flavonoids nanoparticles(BLFN) were prepared by ionic cross-linking method, using turnip polysaccharide as a carrier, ε-polylysine as a crosslinking agent. The particle diameter is about 400 nm, particle size distribution is concentrated and of good dispersibility. After B16 melanoma cells were treated with BLFN, the cell proliferation rate, tyrosinase activity and melanin content were measured respectively, and the effects of BLFN on B16 cells were compared with that of arbutin. Results showed that the tyrosinase activity, the synthesis of melanin and proliferation rate of B16 cells were all negatively related to the drugs concentration. When the concentration is 100 μg/mL, the inhibition rate reached at 69.68%, 62.35% and 59.14% respectively, showing significant difference as compared to arbutin (p <0.05). In conclusion, BLFN is a potential substitute for arbutin which has been proved to be an effective inhibitor for melanin synthesis. Meanwhile, the nanoparticle is a combination of various natural products with lower toxicity, which ensuring broad prospects in its application as whitening products.