基于化学方法制备油酸改性超顺磁性氧化铁纳米粒子

Preparation of Superparamagnetic iron oxide nanoparticles modified with oleic acid based on chemical methods

摘要:

目的 油酸改性超顺磁性氧化铁纳米粒子（O-SPION）被应用于制备高品质的MRI T2阴性造影剂或搭载药物的磁靶向分子探针，不同于传统的物理合成方法，本课题组尝试采过化学方法制备O-SPION，并通过体外实验检测其细胞毒性及细胞透过性。方法 通过共沉淀法制备分散性好、磁响应强的超顺磁性纳米粒子。对油酸的羧基进行活化后，利用缩合反应实现油酸改性SPION的化学合成。采用X-Ray衍射仪、红外光谱仪、激光粒度分布测试仪、透射电镜对产物进行表征。MTT法检测其对人肝癌细胞HepG2的毒性作用，普鲁士蓝染色检测其细胞摄取能力。结果 O-SPION的核心粒径为12?.5 nm的，其具有稳定的化学结构和低表面电势，体外实验证明其低毒或无毒性，且在细胞内的摄取量较原始SPION明显增多。结论 利用化学方法成功合成了O-SPION,为进一步制备高品质MRI造影剂提供实验依据。

Abstract:

Objective Superparamagnetic iron oxide nanoparticles modified with oleic acid (O-SPION) was used to prepare the high quality MRI T2 negative contrast agent or magnetic targeting molecular probe load drugs.Our group attempt to use chemical methods different from traditional physical Synthesis methods to prepare O-SPION, and detect the cytotoxicity and permeability for cells in vitro experiment. Methods Preparating superparamagnetic iron oxide nanoparticles with good dispersity and high magnetic response by coprecipitation methods. Modified SPION with oleic acid by condensation reaction after activating the carboxyl of oleic acid. X-ray diffractometry (XRD), Fourier transform infrared (FT-IR) spectra, Laser grain size measure and Transmission electron microscopy (TEM) were used to measure products. Cell viability was determined by MTT assay with human hepatoma cell line HepG2. Prussian blue staining of the O-SPION assayed its ability of cell uptake. Results The average particle size of O-SPIONs was 12 ?1.5 nm. O-SPION had stable chemical structure and lower surface potential. Cell viability demonstrated that O-SPION showed low toxicity or nontoxicity, and its intracellular uptake was greater than the control group.Conclusion Successfully synthesized O-SPION by using chemical methods, and provided experimental basis for preparing high quality MRI contrast agent.