明胶纳米颗粒的制备及表征

Preparation and Characterization of Gelatin Nanoparticles

摘要:

以B型明胶为原料，戊二醛为交联剂，通过改进的两步去溶剂法制备了较小粒径（约100nm）的明胶纳米颗粒(Gelatin nanoparticles, GNPs)；研究了分散体系浓度、去溶剂化试剂、交联剂用量的影响。结果表明：降低明胶在二次分散体系中的浓度，制得的GNPs的尺寸更小且分布均匀。残留的去溶剂化试剂丙酮必须去除，因为它会使GNPs分散性变差。在明胶质量浓度为8 g/L的分散液中，仅用质量分数为4.5%的戊二醛(与明胶质量比)即可制得稳定的GNPs，其粒径为50~150 nm，PDI （polydispersity index）约为0.14，Zeta电位绝对值< 30 mV。AFM和 TEM测试结果表明GNPs呈光滑球形，为边缘部分比核心更为疏松的非均质结构。

Abstract:

Gelatin nanoparticles (GNPs) of about 100 nm in diameter have been prepared from type B gelatin crosslinked with glutaraldehyde by the modified two-step desolvation process. The effects of the gelatin concentration in dispersions, desolvation solvent (acetone), and the amounts of the cross-linker (glutaraldehyde) have been investigated. The results show that the size and dispersion of the GNPs can be decreased by reducing the content of gelatin in the second dispersed solution before crosslinking. Moreover, the residual acetone in the as-prepared dispersion must be removed completely, since it leads to the forming of larger and less stable particles. Using only 4.5% (w / w gelatin) glutaraldehyde in the 8 g/L (w / v solution) gelatin dispersion can produce stable GNPs with the diameter of 50 ~ 150 nm, a polydispersity index of about 1.4, and Zeta potential < -30 mV. Atomic force microscope (AFM) and transmission electron microscope (TEM) analysis shows that the nanoparticles are spherical morphology and heterogeneous spheres whose edge parts are looser than the cores.