蔗渣木聚糖没食子酸酯-g-MAA/BA的合成及抗癌活性

Synthesis and Anti-cancer Activity of Methacrylic Acid/ Butyl Acrylate grafted onto Bagasse Xylan Gallate

摘要:

以蔗渣木聚糖(BX)为主要原料、没食子酸为酯化剂、三乙胺为催化剂、以N, N-二甲基甲酰胺(DMF)为溶剂，合成了蔗渣木聚糖没食子酸酯。接着以过硫酸铵引发甲基丙烯酸(MAA)/丙烯酸丁酯(BA)与蔗渣木聚糖没食子酸酯共聚，合成了蔗渣木聚糖没食子酸酯-g-MAA/BA酯化接枝共聚物，并考察了合成工艺参数对其取代度(DS)、接枝率(G)和接枝效率(GE)的影响，采用FTIR、SEM、XRD与TG-DTG对产物的结构和性能进行表征。结果表明：在n(BX): n(三乙酰没食子酰氯)=1: 1、m(DMF): m(BX)=9.5: 1、45 ℃反应4 h时，DS为0.64；在n(蔗渣木聚糖没食子酸酯): n(BA/MAA)=1: 2、w(过硫酸铵)(以反应物总质量计)=7%、50 ℃反应8 h时，G和GE分别为23%和67%。采用溴化噻唑蓝四氮唑(MTT)法对蔗渣木聚糖没食子酸酯-g-MAA/BA进行了抗癌活性测试，表明该产物对肝癌细胞的抑制率为28.83%。

Abstract:

Bagasse xylan gallate was synthesized by using bagasse xylan as main raw materials, gallic acid as esterifying agent, triethylamine as activation catalyst and N, N-dimethyl formamide (DMF) as the solvent. The esterification and graft copolymerization of methacrylic acid (MAA)/ butyl acrylate (BA) grafted onto bagasse xylan gallate was carried out by using ammonium persulfate as initiator. The parameters of the process affected on the degree of substitution (DS), grafting rate (G) and grafting efficiency (GE) were investigated. The structure and properties of the product were characterized by FTIR, SEM, XRD and TG-DTG. The results showed that the DS was 0.64 under the conditions of n(bagasse xylan): n(trisacetyl-galloylchloride)=1: 1, m(DMF): m(bagasse xylan)=9.5: 1, the esterification reaction temperature 45 ℃ for 4 h. The grafting rate and grafting efficiency of the product was 23% and 67% under n(bagasse xylan gallate): n(BA/MAA)=1: 2, with the ammonium persulfate dosage being 7% (the total reactant mass), reaction temperature 50℃ for 8 h. The product of anti-cancer activity was evaluated against some tumor cell lines by using a methylthiazolyldiphenyl-tetrazolium bromide (MTT) method. The results showed that the inhibition rate on cancer cells of the liver reached to 28.83%.