苯并咪唑类衍生物荧光增强型PO43-荧光分子探针

Fluorescence-enhanced Probe for PO43– Based on Benzimidazole Derivative

摘要:

以对苯二胺、氯乙酰氯、苯并咪唑和溴代正戊烷为原料合成了一个荧光分子探针1,4-苯基-双[(氨甲酰甲基)-1-戊基苯并咪唑盐酸盐][M]，收率为80.1%。用核磁共振氢谱和红外光谱对其结构进行了表征，并用荧光光谱在水溶液（HEPES缓冲溶液，pH=7.4）中研究了其对阴离子的识别性能。结果表明：M对PO43–具有快速、直接、较高的选择性识别性能，在1.0?0–5 mol/L的M溶液中滴加9.0?0–4 mol/L 的PO43–后荧光发射波长红移36 nm，荧光强度增强，与PO43–结合后的荧光发射强度不受其它常见阴离子的干扰，是一种很好的荧光材料。

Abstract:

A benzimidazole-based fluorescent molecular probe phenyl-1,4-bis [(carbamoylmethyl)-1-pentylbenzimidazole hydrochloride)][M] was synthesized by using p-phenylenediamine, chloroacetyl chloride, benzimidazole and 1-brompentane as raw materials, yield 80.1%. Structure of M was identified by nuclear magnetic resonance spectroscopy and infrared spectroscopy, studying its determination of anions in aqueous solution(HEPES buffer solution, pH=7.4) by fluorescence spectroscopy. The results indicated probe M displayed rapidity, high selectivity, directness for the determination of phosphate inos, the addition of 9.0?0–4 mol/L PO43– in the 1.0?0–5 mol/L M solution led to a 36 nm red-shift in fluorescence maximum, an fluorescence enhancement, and M recognizes phosphate inos without interference from other common anions. All date show that M is a kind of good fluorescence materials.