多离子识别荧光探针的合成及其机理

Synthesis and Mechanism of Multi-ion Recognition Fluorescence Probe

摘要:

以席夫碱官能团作为连接臂、香豆素和罗丹明作为发色基团，合成了一种同时含罗丹明和香豆素的席夫碱化合物L ，用1HNMR、13CNMR及HRMS对探针的结构进行了表征，并对其识别Ca2+、Cd2+和Al3+的机理进行了考察。结果表明：L在乙醇体系中对Ca2+、Cd2+和Al3+可选择性识别，并且对于其它金属离子具有一定的抗干扰能力。L及L+离子在pH=7～ 12有明显的荧光增强。HRMS和Job曲线实验结果表明：L可分别与Ca2+、Cd2+、Al3+形成摩尔比为1:1、2:1和2:1的配合物。

Abstract:

A Schiff base compounds containing both rhodamine and coumarin L was synthesized by using Schiff base functional groups as linker, coumarin and rhodamine as chromogenic groups. The structure of the probe was characterized by 1HNMR, 13CNMR and HRMS. The recognition mechanism of Ca2+, Cd2+ and Al3+ were also investigated. The results show that L can selectively recognize Ca2+, Cd2+ and Al3+ in ethanol system and have some anti-interference ability over other metal ions. L and L+ions at pH 7～12 have significant fluorescence enhancement effect. High resolution mass spectrometry and the Job curve show that L can form complexes with Ca2+, Cd2+ and Al3+ in molar ratios of 1:1, 2:1 and 2:1, respectively.