负载锌酞菁的二氧化硅纳米载体在光动力疗法的应用

Application of ZnPc loaded macroporous silica nanocarrier in the photodynamic therapy

摘要:

本文在传统介孔二氧化硅合成的基础上，以Ca2+为扩孔剂，利用层层自组装技术包裹CS（壳聚糖）/ HA（透明质酸）层，制备得到可降解的大孔二氧化硅 (DMSNs@CS/HA)，在DMSNs@CS/HA上负载疏水性药物锌酞菁(ZnPc)，得到负载锌酞菁的二氧化硅纳米载体DMSNs@CS/HA@ZnPc。将其应用于光动力学抗肿瘤研究。采用SEM、TEM、FTIR、XRD，紫外吸收光谱图，N2吸附等温线、荧光成像技术对DMSNs@CS/HA和DMSNs@CS/HA@ZnPc进行了物相、形貌、孔径分布、红外结构测试、肿瘤光动力学治疗像实验。结果表明，当DMSNs@CS/HA纳米载体的质量浓度为50mg/L，Hela细胞的相对存活率高达93.2%，而负载ZnPc并在近红外光照10min后，细胞存活率则锐减到36.2%。细胞存活率及其荧光成像实验均表明DMSNs@CS/HA纳米载体具有良好的生物相容性，可有效负载ZnPc，并应用于近红外照射下光动力学抗肿瘤。

Abstract:

Degradable macroporous silica nanoparticle (DMSNs@CS/HA) was prepared from traditionally prepared mesoporous silica using Ca2+ as pore enlarged reagents, and using good biocompatible chitosan (CS) and hyaluronic acid (HA) through layer-by-layer self-assembly technique. The obtained multifunctional nanocarrier was used to load hydrophobic drugs (zinc phthalocyanine) for photodynamics treatment of tumor. Herein, the phase, morphology, pore size distribution, infrared spectroscopy, photodynamics antitumor effect and fluorescence imaging experiment of the as-prepared DMSN@CS/HA were investigated. The survival rate of Hela cells incubated with DMSNs@CS/HA at the concentration of 50 mg/L, reaches up to 93.2%. After loading ZnPc and subjected to the near infrared ray (NIR) irradiation for 10 min, the survival rate sharply decreases to 36.2%. The MTT assay and fluorescence imaging suggest that DMSNs@CS/HA nanocarrier has good biocompatibility, can effectively load ZnPc. And DMSNs@CS/HA@ZnPc shows the excellent photodynamics therapeutic effect under NIR radiation.