大孔吸附树脂分离纯化猪毛菜总黄酮及其抗氧化活性

Separation and Purification of Total Flavonoids from Salsola collina with Macroporous Resins and Evaluation of Antioxidant Activities

摘要:

采用大孔吸附树脂分离纯化猪毛菜总黄酮，并对纯化后的总黄酮进行体外抗氧化活性研究。通过考察影响树脂静态和动态吸附与洗脱的主要因素，确定猪毛菜总黄酮分离纯化优化工艺条件。静态吸附实验表明，AB-8树脂分离纯化效果较好并且吸附符合Langmuir和Freundlich方程。动态吸附和解吸的最佳工艺条件为上样液质量浓度1.25mg/mL，pH4.5，上样流速2mL/min，上样量2.5BV，洗脱剂80%乙醇，洗脱流速1mL/min，洗脱剂用量4BV。所得洗脱液中黄酮纯度从纯化前的10.2%可增加到纯化后51.89%，回收率为84.43%。体外实验表明，纯化后的黄酮可以清除羟自由基和氧自由基并有较好的还原力。本实验中最优纯化条件简单、可行，可为工业化生产提供参考。结果表明纯化后的黄酮可以作为一种潜在的天然抗氧化剂。

Abstract:

Separation and purification of total flavonoids from Salsola collina using macroporous resins and the antioxidant activities in vitro of purified flavonoids were studied. Critical factors that influence the static and dynamic adsorption and desorption of total flavonoids were selected and optimized. The static tests indicated that AB-8 resin was appropriate and its adsorption data were well fitted to the Langmuir and Freundlich isotherms. The optimal conditions for dynamic adsorption and desorption were sample concentration of 1.25mg/mL, pH 4.5, flow rate of 2mL/min, sample loading amount of 2.5BV, and complete desorption with 4BV of 80% ethanol at 1mL/min. After the optimized purification, the purity of flavonoids was improved from 10.2% to 51.89%, and the recovery yield was 84.43%. The antioxidant tests revealed that the purified ?avonoids could scavenge hydroxyl and oxygen radicals and possessed strong reducing power. The optimum conditions for purifying flavonoids are simple and feasible, which could provide experimental basis for the industrial production. The results have demonstrated that the purified ?avonoids from Salsola collina can be used as a potential source of natural antioxidant.