杭白菊中多酚物质的提取工艺及其抗氧化性研究

Response surface methodology for optimization of extracting polyphenol from Chrysanthemum morifolium Ramat.and antioxidant evaluation

摘要:

该研究以杭白菊为材料，以多酚提取率为评价指标，在SDS浓度、液固比、超声时间、超声温度单因素试验基础上，采用响应面法优化了杭白菊多酚提取工艺，并对杭白菊多酚成分进行了分析。同时测定了多酚对总还原力、DPPH自由基、OH自由基的清除能力。结果表明：杭白菊多酚提取最佳工艺条件为SDS溶液浓度1.50%、液固比为25mL/g、超声时间24min、超声温度65℃。在此条件下，多酚提取率预测值为12.591mg/g，实际为12.412mg/g，理论与预测值的相对误差为1.442%。杭白菊多酚的总还原力、清除OH自由基ED50值分别为9.00、148.862μL，强于VC（20.00、219.050μL）和BHT（415.00、487.46μL）；清除DPPH自由基能力（ED50为8.201μL）弱于VC强于BHT（ED50为86.061μL）。应用高效液相色谱法分析其成分，结果显示：杭白菊多酚主要成分为异绿原酸A，含量为5.8704mg/g,其次为木犀草苷含量为3.1306mg/g, 绿原酸含量为3.1025mg/g。该提取工艺稳定合理，准确可靠，是提取杭白菊多酚的可行方法。该研究结果为杭白菊多酚成分的进一步开发利用奠定了基础。

Abstract:

In order to determine the best extraction technology of polyphenol from Chrysanthemum morifolium Ramat.and evaluate their antioxidant activity, and analyze the chemical composition, SDS concentration, liquid-solid ratio, ultrasonic time and ultrasonic temperature as single factor were tested and then the extraction process of the polyphenol from Chrysanthemum morifolium Ramat.was optimized by response surface designs based on single factor experiments. In addition, the antioxidant activity of polyphenol from Chrysanthemum morifolium Ramat. was measured using the total reducing power, l1-diphenyl-2-picryhydrazyl radical (DPPH) and hydroxyl radical (OH) scavenging. The results showed that the polyphenol of optium extraction conditions of Chrysanthemum morifolium Ramat.were as follows: SDS concentration 1.50%, liquid-solid ratio 25 mL/g, ultrasonic time 24 min and ultrasonic temperature 65℃. Under these conditions, an extraction rate of 12.412 mg/g for polyphenol from Chrysanthemum morifolium Ramat.was obtained, compared with the predictive value of 12.59 mg/g, the relative error was 1.442%. The ED50 values of the total reducing power, scavenging OH radical were 9.00, 148.862μL, stronger than that of VC (20.00, 219.050μL) and BHT (415.00, 487.46μL); The scavenging DPPH radical (ED50 was 8.201μL) was weaker than that of VC, but stronger than that of BHT (ED50 was 86.06μL). The results of Chromatography analysis with HPLC show that isochlorogenicacid A, galuteolin, chlorogenic Acid, are the main components existing in polyphenols.The content of 3 respective components were 5.8704 3.1306 and 3.1025mg/g. The extraction process was stable and reasonable, accurate and reliable, and it can be used to extract the polyphenol from Chrysanthemum morifolium Ramat.The result will provide basis for the further development and utilization of polyphenols of Chrysanthemum morifolium Ramat..