响应面法优化闪式提取罗汉松总黄酮及抗氧化活性研究

Optimization of Flash Extraction of Flavonoids from Leaves of Podocarpus Macrophyllus by Response Surface Method and Antioxidant Activity

摘要:

本研究对罗汉松总黄酮提取工艺及其抗氧化活性进行了研究，采用闪式提取法提取罗汉松总黄酮，并通过单因素法和响应面优化方法，同时考察了罗汉松总黄酮对DPPH•、O2-•和•OH自由基清除作用。结果表明，闪式提取罗汉松总黄酮最佳工艺条件为：乙醇浓度71 %、料液比1:36 (g·mL-1)、提取时间53 s、提取电压为75 V时，罗汉松总黄酮提取率为8.147 %。罗汉松总黄酮对DPPH•、O2-•和•OH具有明显的清除能力，最大清除率分别达到87.13%、58.24%和67.56%，为罗汉松的综合开发利用提供了参考。

Abstract:

The flash extraction of total flavonoids from the leaves of Podocarpus macrophyllus has been optimized by response surface method, and antioxidant activities were studied. The design of extraction of total flavonoids is from Box-Behnken central composite experiment，at the same time, the scavenging activities on DPPH•、O2-•and •OH free radical of the total flavonoids of Podocarpus macrophyllus were measured. The results show that the extraction rate of total flavonoids of Podocarpus macrophyllus was 8.147%，and the optimum conditions were obtained as follows: the ethanol concentration was 71%，the material liquid ratio 1:36(g·mL-1)，the extraction time is 53s and the voltage is 75 V. Total flavonoids of Podocarpus macrophyllus have obvious scavenging ability to DPPH•、O2-•and •OH，and the maximum clearance rates were 87.13%, 58.24% and 67.56%，which can provide a foundation for the development of Podocarpus macrophyllus.