COS-g-PEI修饰纳米银粒子的制备及其抗菌性研究

Synthesis and Studies on Antimicrobial Property of Ag NPs Protected by COS-g-PEI

摘要:

首先以离子液体1-丁基-3-甲基咪唑氯盐（[BMIM]Cl）为反应介质和催化剂，羰基二咪唑为偶联剂，均相合成了壳寡糖接枝聚乙烯亚胺共聚物 (COS-g-PEI)；其次，以COS-g-PEI为稳定剂，硝酸银为前驱体，硼氢化钠为还原剂，采用原位还原法制备了COS-g-PEI修饰纳米银复合粒子（Ag NPs@-COS-g-PEI）。采用IR、1H-NMR对聚合物结构进行表征，结果证实成功合成了COS-g-PEI 接枝共聚物；UV-Vis、TEM结果表明，成功制备了稳定的Ag NPs@-COS-g-PEI 复合粒子。通过滤纸片法和抑菌圈法测试了COS-g-PEI 及Ag NPs@-COS-g-PEI对金黄色葡萄球菌和大肠杆菌的抗菌性能，Ag NPs@-COS-g-PEI的抑菌圈直径均比COS-g-PEI大，结果表明，Ag NPs与COS-g-PEI有机结合大大提高了抗菌性能。

Abstract:

The stable Ag NPs protected by chitosan oligosaccharide grafted with polyethylenimine (COS-g-PEI) were prepared, and the antibacterial properties were studied. First, COS-g-PEI was synthesized using ionic liquid 1-butyl-3-methyl imidazolium chloride（[BMIM]Cl）as solvent and catalyst, and 1, 1-carbonyldiimidazole as coupling reagent. Then, Ag NPs@-COS-g-PEI composite particles were prepared by in-situ reduction method using COS-g-PEI as stabilizer, sodium borohydride as reductant. The results of IR and 1H-NMR showed that COS-g-PEI was prepared successfully. And the results of UV-Vis and TEM showed that the stable Ag NPs@-COS-g-PEI composite particles were prepared. The antimicrobial properties of COS-g-PEI and Ag NPs@-COS-g-PEI for staphylococcus aureu and escherichia coli were evaluated by Kirby-Baner test and oxford cup method. Ag NPs@-COS-g-PEI showed the larger diameter of inhibiting zone than COS-g-PEI against staphylococcus aureu and escherichia coli no matter by Kirby-Baner test or oxford cup method. It is suggested that the antibacterial properties of COS and Ag NPs are combined together to give higher antibacterial ability.