六氢β-酸环糊精包合物抑菌活性及其稳定性研究

Study on Antimicrobial Properties and Stability of Hexahydro-β-acids-Cyclodextrin Inclusion Compound

摘要:

以金黄色葡萄球菌和大肠杆菌为供试菌，研究六氢β-酸环糊精包合物抑菌活性及其稳定性。采用滤纸片法测定7种环糊精包合物抑菌活性，二倍稀释法测定六氢β-酸-2,6二甲基-β-CD、六氢β-酸-2-甲基-β-CD和六氢β-酸-羟丙基-β-CD包合物的最低抑菌浓度，并研究了温度、pH、紫外线对前述3种包合物抑菌稳定性的影响。结果表明：7种环糊精包合物均有不同程度的抑菌活性，其中六氢β-酸-2,6二甲基-β-CD、六氢β-酸-2-甲基-β-CD和六氢β-酸-羟丙基-β-CD包合物抑菌效果最好，最低抑菌浓度均为8.0 μg/mL。经酸碱处理后，与未处理组比较，在pH>6的条件下，3种包合物的抑菌活性增强，在pH<6的条件下，其抑菌活性减弱；温度和紫外线对3种包合物抑菌活性无影响。

Abstract:

Abstract: The antimicrobial properties and stability of hexahydro-β-acids cyclodextrin inclusion complex were investigated by using staphylococcus aureus and Escherichia coli as tested bacteria in this study. Antimicrobial properties of seven kinds of cyclodextrin inclusion complex were determined by filter paper method, furthermore, the minimum inhibitory concentration of three of them against these bacterias, namely hexahydro-β-acids-2,6-dimethyl-β-cyclodextrin, hexahydro-β-acids-2-methyl-β-cyclodextrin and hexahydro-β-acids-hydroxypropyl-β-cyclodextrin were determined by doubling dilution method. Meanwhile, the effects of temperature, pH, ultraviolet on the antimicrobial stability of the three kind of inclusion complex were also investigated. The results suggested that all of the inclusion compounds had antimicrobial activity, hexahydro-β-acids-2,6-dimethyl-β-cyclodextrin, hexahydro-β-acids-2-methyl-β-cyclodextrin and hexahydro-β-acids-hydroxypropyl-β-cyclodextrin exhibited the best inhibitory effect, the minimum inhibitory concentrations of the three kind of inclusion complex were all 8.0 μg/mL. Compared with untreated group, the antibacterial activity of 3 kinds of inclusion compounds enhanced under the condition of pH>6, whereas decreased under the condition of pH<6; temperature and ultraviolet had no effect.