微波辅助骨碎补黄酮的提取及抗氧化活性研究

Optimization of process for microwave-assisted extraction of flavonoids from drynaria rhizome and its antioxidant activity

摘要:

摘 要：本文利用微波辐射所产生的机械空化破碎植物细胞的细胞壁，利用响应面优化结合BBD实验设计，建立骨碎补黄酮提取率与提取因素之间定性与定量的标准，确定因素之间的交互作用与最佳水平组合；以骨碎补黄酮清除硫酸亚铁和过氧化氢在水杨酸产生的羟基自由基作为评价抗氧化活性指标。为骨碎补黄酮应用及功能产品的开发提供理论参数及数据指标。实验结果表明，骨碎补黄酮最佳提取条件为料液比1:35 g/mL，微波功率400 W、微波时间9 min，黄酮得率最高为5.57%；抗氧化活性实验表明，骨碎补能够清除一部分羟基自由基，在质量浓度为2.50 mg/mL时，对应最大的为清除率为10.12%。以上实验结果表明，通过响应曲面确定了提取率与影响因素之间的二次多项函数模型，为定量提取提供了理论依据，同时说明骨碎补黄酮具有体外抗氧化活性，以期能够应用于食品及药品行业，作为天然的防腐抗氧化试剂进行深层次研究与开发。

Abstract:

Abstract:In this work, Extraction process of flavonoids from the drynaria rhizome by using microwave radiation produced by mechanical cavitation broken cell walls of plant cell and with response surface and Box-Benhnken design method was optimized qualitative and quantitative standards between yields of flavonoids and influence factors to confirm interactions between factors and best level combination. The antioxidant activty was estimated by the scavenging activitiy against hydroxyl radical with the oxidation of ferrous sulfate-salicylic acid-hydrogen for rhizoma drynariae flavonoids application and the development of functional products provide theoretical parameters and data index. The results showed that the best extractionof flavonoids was ration of liquid to materials 、microwave power micrwavere and microwave time（1:35 g/mL、400W、9min） and achieved the maximum yield of 10.12% (2.50 mg/ml). The above experimental results show that extraction yield was determined by the response surface and quadratic multinomial function model between influence factors, which provides a theoretical basis for the extraction of quantitative and rhizoma drynariae flavonoids has antioxidant activity in vitro which can be used in food and pharmaceutical industry for a natural preservative oxidation reagents by going deep into research and development.