漆酶催化聚合对氨基苯酚

Enzymatic Polymerization of 4-aminophenol Catalyzed by Laccase

摘要:

漆酶在pH 5.0的醋酸盐缓冲溶液中催化聚合4-氨基苯酚，微型反应器中40℃持续反应10h后得到黑褐色粉末。通过UV-Vis、FT-IR、1HNMR、MALDI-TOF MS、TGA和DSC对产物的结构和性能进行表征。结果表明：所得聚合物在氯仿中不溶，在丙酮、四氢呋喃、N,N-二甲基甲酰胺和二甲基亚砜中均有良好的溶解性能。根据UV-Vis、FT-IR和1HNMR分析得知聚合物中酚羟基保留，氨基参与反应，苯环上的氢部分被取代，并且推测了可能的聚合物结构。根据MALDI-TOF MS、TGA和DSC结果分析，聚合物为低聚物，相对分子质量分布在300Da到1500Da之间，聚合度从3到14。产物的分解温度为223℃，具有良好的耐热性能。

Abstract:

Enzymatic polymerization of 4-aminophenol was conducted using laccase enzyme from Trametes hirsute. The polymerization reaction was carried out in acetate buffer solution ( pH 5.0) at 40℃. After 10h, black solid power was obtained. The molecular weight of the product was determined by MALDI-TOF MS. UV-Vis, FT-IR, DSC and TGA were employed to investigate the structure and thermal behavior of produced poly(4-aminophenol). The possible structure of polymer was speculated. The molecular weight of poly（aminophenol）ranges from 300Da to 1500Da, the degree of polymerization are from 3 to 14. Thermal analysis showed that the decomposition temperature of polymer was 223℃，indicating that the polymer have excellent heat resistance.