谷氨酰转肽酶细胞合成L-谷氨酰正丙胺的研究

Synthesis of Propyl-Ｌ-Glutamine by glutamyltranspeptidase cell

摘要:

摘要：利用谷氨酰转肽酶细胞合成L-谷氨酰正丙胺，采用L-谷氨酰肼和正丙胺作为底物，考察底物浓度、温度、pH、催化剂用量和底物物质的量对合成L-谷氨酰正丙胺的影响。研究结果表明：温度为37 ℃、pH为10、L-谷氨酰肼浓度为0.4 mol/L，正丙胺浓度为3 mol/L，产物L-谷氨酰正丙胺的产率为97.9%，L-谷氨酰正丙胺收率为85.3%，L-谷氨酰正丙胺纯度为99.8%。谷氨酰转肽酶细胞能有效合成L-谷氨酰正丙胺。

Abstract:

Abstract: Synthesis of propyl-L-glutamine by glutamyltranspeptidase cells was investigated. With L-glutamlhydrine and propylamine as substrate, the factors such as substrate concentration, pH and temperature were investigated. The results indicated that when the concentration of L-glutamlhydrine was 0.4 mol/L, the concentration of propylamine was 3 mol/L, reaction temperature was 37ºC, and pH was 10, the yield of obtained propyl-L-glutamine reached 97.9%.The yield of propyl-L-glutamine was 85.3%. The purity of propyl-L-glutamine was 99.8%. Synthesis of propyl-L-glutamine by glutamyltranspeptidase cells was effective.