铁棒锤总生物碱、3-乙酰乌头碱和宋果灵抗肿瘤活性研究

Study on AntitumorActivity of Total Alkaloids, 3-acetylaconitine and Songorine from Aconitum szechenyianum Gay.

摘要:

本文从铁棒锤总生物碱中提取分离得到3-乙酰乌头碱和宋果灵，应用MTT比色法研究药物的细胞毒型、细胞形态学实验方法观察细胞形态、流式细胞术分析细胞凋亡和细胞周期，激光共聚焦从形态上分析细胞的凋亡等多方面对其抑制人肝癌细胞的生理活性进行研究。3-乙酰乌头碱和宋果灵的抗肿瘤细胞研究表明，随着浓度的逐步增加，其抗肿瘤效果逐步增强；药物作用于人肝癌细胞后，细胞数量明显减少，具有明显的抑制HepG2生长的能力，并且使细胞发生早期凋亡和晚期凋亡的比率增大，随处理时间的延长细胞G1期细胞逐渐增多，G2期和S期细胞逐渐减少。3-乙酰乌头碱和宋果灵的体外抗肿瘤实验研究初步证实了其具有抑制肿瘤细胞增殖、诱导肝癌细胞凋亡的作用，显示3-乙酰乌头碱和宋果灵可能成为新的肝癌细胞凋亡诱导剂，为寻找新型抗肿瘤药物的先导化合物奠定了基础。

Abstract:

In this paper, 3-acetylaconitine and songorine were extracted and separated from the total alkaloids of Aconitum szechenyianum Gay., and their physiological activity on inhibition of human liver cancer cells were studied by MTT method to study the cytotoxic patterns of drugs, cell morphology experiment to observe cell morphology, flow cytometry to analysis cell apoptosis and cell cycle, and laser confocal microscopy to analysis cell apoptosis. The antitumor experiments of 3-acetylaconitine and songorine showed that the antitumor effects were gradually enhanced with the increase of the concentration. The number of cells was significantly reduced, and the growth of HepG2 was inhibited significantly after the drugs act on HepG2. In addition, the ratio of cells early apoptosis and late apoptosis increased, and the G1 phase cells gradually increased, but G2 and S phase cells gradually decreased with the prolonging of treatment time. The antitumor research of 3-acetylaconitine and songorine in vitro had confirmed their effects on inhibition tumor cell proliferation and induction liver cancer apoptosis, which showed that 3-acetylaconitine and songorine could become the new cell apoptosis inducers of liver cancer, and laied the foundation for new antitumor drug lead compounds.