线粒体内活性硫有机荧光小分子探针研究进展

Research Progress of Organic Fluorescent Small Molecule Probes for Reactive Sulfur Species in Mitochondria

摘要:

线粒体是细胞内活性硫物种(Reactive sulfur species, RSS)的主要来源。线粒体内RSS在生命体的生理和病理过程中发挥着至关重要的作用。该文综述了近年来有机单、双光子线粒体内H2S、SO2、巯基生物分子、多硫化氢等RSS荧光探针研究现状，重点讨论了荧光探针选择性、灵敏度、识别机理、细胞毒性以及生物监测成像等方面的性能，并提出这类荧光探针存在的问题以及未来的发展趋势，旨在为构建新型线粒体荧光探针和进一步剖析线粒体内RSS的细胞学功能提供重要的理论和实践意义。

Abstract:

Mitochondria are a major source of cellular reactive sulfur species (RSS). The RSS play crucial roles in physiological and pathological processes of life. In this paper, we review the recent research status of organic one and two-photon fluorescent probes for RSS in mitochondria, such as H2S, SO2, biothiols, H2Sn , etc. The properties of fluorescent probe selectivity, sensitivity, recognition mechanism, cell toxicity and biological monitoring imaging are emphatically discussed. Moreover, the existing problems and future development of this kind of fluorescent probes are proposed．This review is significantly meaningful in theory and practice for the construction of novel mitochondrial fluorescent probes and further dissection of the cellular function of RSS.