木犀草素-Zn(II)配位印迹聚合物的制备及在箬叶黄酮碳苷分离中的应用

Preparation of Luteolin-Zn(II) Coordinate Molecularly Imprinted Polymers for The Separation of C-glycosyflavones from Indicalamus leaf

摘要:

以箬叶黄酮碳苷母核木犀草素为虚拟模板分子，结合Zn(II)的配位作用，以丙烯酰胺为功能单体，N，N?-亚甲基双丙烯酰酯胺为交联剂，以乙醇为溶剂制备木犀草素-Zn(II)配位印迹聚合物，将此聚合物与固相萃取相结合，分离醇水体系中的箬叶黄酮碳苷。结果表明 ，当n（模板分子）﹕n（功能单体）﹕n（交联剂）=1:5:30时，配位印迹聚合物具有最好的吸附性能，对醇相中木犀草素的吸附容量可达103.4mg?g-1，是未配位印迹聚合物吸附容量的1.71倍，空白印迹聚合物的13.75倍。采用木犀草素-Zn(II)配位印迹聚合物分离醇水相中箬叶黄酮碳苷，经1次分离后，荭草苷、异荭草、牡荆苷和异牡荆苷的回收率分别为91.95%、80.87%、60.43%和63.63%，含量相比粗提物分别提高55.46、48.78、36.45和38.38倍，4种黄酮碳苷总含量由提取前的0.70%提高32.68%。

Abstract:

With the Zn2 coordination ，the luteolin-Zn(II) coordinate molecularly imprinted polymers(MIPs) were synthesized using luteolin as template, acrylamide(AM) as monomer，N，N?- methylene bis ester amide(MBA) as cross-linker and ethanol as solution. And then C-glycosyflavones from Indicalamus leaf in alcohol-water system were separated by solid phase extraction using MIPs. The results showed that when n(luteolin-Zn)﹕n(AM)﹕n(MBA)=1:5:30，the obtained MIP possessed the highest adsorption capacity of luteolin from methanol phase with a value of 103.4mg?g-1，that is 1.71 times of MIP without coordination，13.75 times of blank polymer. The MIP can revealed selective enrichment capability of C-glycosyflavones from Indicalamus leaf in alcohol-water system, the recovery rates of Orientin, Isoorientin, Vitexin and Isovitexin are 91.95%、80.87%、60.43% and 63.63%, the contents are 55.46、48.78、36.45 and 38.38 times of that in crude extract, the total content of the four kinds of C-glycosyflavones is from 0.70% to 32.68%.