三元有机-钴大环化合物的组装及其识别性能

Assembly of Co-based organic-metal cyclohelicate compound for recognition of glutathione

摘要:

以5-氨基-1,3-苯二甲酸为母体，通过系列衍生引入苄基和喹啉基团合成配体HL，再与钴离子（Co2 ）自组装构筑了有机-钴三元大环化合物HL-Co。利用电喷雾质谱和紫外光谱研究了HL-Co对谷胱甘肽的识别过程与传感作用。研究结果表明，HL-Co在溶液中形成稳定的[3 3]大环结构，并且能够1:1包合谷胱甘肽分子。通过紫外滴定测试发现，当向HL-Co中加入谷胱甘肽后，在320 nm处的吸光度逐渐增加，而425 nm处吸光度逐渐减弱，直至平衡，等吸收点为357 nm。 而且，采用Benesi-Hildebrand数据拟合得到平衡常数log K(GSH)=4.41，对谷胱甘肽识别的最低检测限达到1.0?10-5 mol/L，实现了对谷胱甘肽的有效识别。

Abstract:

A novel organic-cobalt ternary macrocyclic compound HL-Co was constructed by cobalt ion with (N,N′)-5-(dibenzylamino)-N1(E),N′3(E)-bis(quinolin-2-ylmethylene)isophthalohydrazide, which was synthesized by introduction of quinoline and dansylamide groups into 5-aminoisophthalic acid. The recognition process of glutathione was studied with HL-Co by electrospray ionization mass spectrometry (ESI-MS) and ultraviolet spectroscopy (UV-vis). The results show that HL-Co forms a stable [3 3] macrocyclic structure in solution and can encapsulate glutathione at molar ratio of 1: 1. The absorbance at 320 nm was gradually increased when the glutathione was added to the solution of HL-Co, and the absorbance at 425 nm gradually decreased until the equilibrium. The isobestic point at 357 nm was obtained. An equilibrium constant log K(GSH) of 4.41 was calculated by fitting of Benesi-Hildebrand data. The minimum detection limit of glutathione was 1.0?10-5mol/L. It indicated that HL-Co has good recognition property on glutathione.