葡糖醋杆菌G31产细菌纤维素的结构表征及性能测试

Structure Characterization and Performance Test of Bacterial Cellulose Produced by Gluconacetobacter xylinum G31

摘要:

以葡糖醋杆菌G31为菌种，用HS培养基发酵制备细菌纤维素（BC）。利用蒽酮比色法、酶水解法初步证明发酵产物为纤维素，采用FTIR、SEM、XRD、CP/MAS 13C-NMR、DSC和TG对BC的形貌和结构进行表征并对其进行性能测试。结果显示：发酵产物具有超细三维网状结构，属于结晶度为92.42%的纤维素Ⅰ型，具有良好的持水性和拉伸性。同时对其分子量进行测定，Mw为5737，属于低分子量BC，其氧气透过率和水蒸气透过率分别为913.55?3.23 cm3/m2?24h?0.1MPa和656.15?3.92 g/m2?24h，表明该BC具有更好的锁水能力，为其在面膜领域的应用奠定了基础。

Abstract:

The bacterial cellulose was fermented by Gluconacetobacter xylinum G31 strain in HS medium (Hestrin Schramm). The fermentation products were preliminarily proved to be cellulose by anthrone colorimetry method and enzymatic hydrolysis method. The morphologies and structures of BC were characterized by FTIR, SEM, XRD, CP/MAS 13C-NMR, DSC and TG and their properties were tested. The results showed that the fermentation product was bacterial cellulose.It had an ultrafine three-dimensional network structure and belonged to cellulose type I with a crystallinity of 92.42%, and had good water-holding and stretchability. At the same time, its molecular weight was measured (Mw=5737) and showed that it belongs to low molecular weight BC. Its oxygen transmission rate and water vapor transmission rate were 913.55?3.23 cm3/m2?24h?0.1MPa and 656.15?3.92 g/m2?24h respectively, indicating the BC has better water-holding capacity and lays a foundation for its application in the mask field.