双水相法回收异戊酰螺旋霉素生产废水中的螺旋霉素

Recovery of spiramycin from isovalerylspiramycin production wastewater by aqueous two-phase extraction

摘要:

建立了乙醇- K2HPO4双水相体系萃取螺旋霉素的方法，对测得的双水相体系的双节线数据进行拟合，并系统研究了K2HPO4浓度、乙醇浓度、螺旋霉素初始浓度、萃取温度和体系pH对分配系数和萃取率的影响。结果表明，双水相萃取螺旋霉素是自发进行、吸热熵增的过程。当K2HPO4质量分数为20%，乙醇质量分数为16%，萃取温度为25℃，体系pH为9.2~9.5时，分配系数可达36.66以上，萃取率可达97.11%以上。其中，当体系"pH = 9.5" 时，分配系数达到47.52，萃取率达到97.97%。乙醇-K2HPO4双水相体系萃取螺旋霉素的纯化倍数及萃取率高，而且所用成相物质乙醇和K2HPO4可以回收重复使用，避免了二次污染，为处理含螺旋霉素的异戊酰螺旋霉素生产废水提供了新的方法。

Abstract:

A new method of using ethanol-K2HPO4 aqueous two-phase system for extracting spiramycin was established. The optimum equation suitable for bimodal data fitting of the system was selected. The influence of K2HPO4 mass fraction, ethanol mass fraction, spiramycin concentration, extraction temperature and pH on the partition coefficient and extraction efficiency was investigated. The experimental results showed that the extraction process of spiramycin was spontaneous, endothermic and entropy increasing. Under the condition of K2HPO4 mass fraction of 20%, ethanol mass fraction of 16%, extraction temperature of 25℃, and pH of 9.2~9.5, the partition coefficient and extraction efficiency of spiramycin were above 36.66 and 97.11%, respectively. When pH = 9.5, the partition coefficient reached 47.52 and the extraction efficiency reached 97.97%. The purification factor and extraction efficiency of spiramycin using ethanol-K2HPO4 aqueous two-phase extraction were high. The phase-forming materials ethanol and K2HPO4 can be recycled for reuse, avoiding secondary pollution. A new method was provided for the treatment of isovalerylspiramycin production wastewater containing spiramycin.