杭白菊浸膏中香气活性成分分析

Identification of Aroma-active Compounds from Hangzhou Chrysanthemum moriflium Ramat extract

摘要:

采用固相微萃取法（SPME）和溶剂辅助风味蒸发（SAFE）两种前处理方法，结合气相色谱-质谱联用（GC-MS）对杭白菊浸膏的挥发性成分进行了分析，同时采用气相色谱-嗅觉检测技术（GC-O）和芳香稀释法（AEDA）对浸膏中的香味活性成分进行了分析研究。结果表明，两种前处理方法结合气质联用仪共检测出119种化合物，醇类30种，酸类6种，酯类28种，醛4种，酮12种，烃类36种，其他3种，其中萜烯类及其的含氧衍生物为主要化合物，同时SAFE较SPME法分析得到的化合物更多，更有优势；GC-O结合AEDA分析共筛选出35种香气活性成分，风味稀释因子（FD因子）较高的活性成分有芳樟醇(2187)、α-石竹烯(729)、苯乙酸乙酯(729)、己酸乙酯(729)、异戊酸乙酯(729)、α-蒎烯(243)、α-松油醇(243)、异戊酸(243)等。

Abstract:

The volatile components of Hangzhou Chrysanthemum moriflium Ramat extract were analyzed by solid phase microextraction (SPME) and solvent-assisted flavor evaporation (SAFE) combined with gas chromatography-mass spectrometry (GC-MS). The aroma active compounds in the extract were further analyzed by gas chromatography-olfactometry (GC-O) in aromatic dilution method (AEDA). The results showed that a total of 119 compounds were detected in the two pretreatment methods, 30 alcohols, 6 acids, 28 esters, 4 aldehydes, 12 ketones, 36 hydrocarbons, and 3 others. Monoterpenes, sesquiterpenes, and their oxygen-containing derivatives are the main compounds. SAFE is suitable for extraction than SPME. A total of 35 aroma active ingredients were identified. The aroma active compounds with higher flavor dilution factor (FD factor) were linalool (2187) , α-caryophyllene (729), ethyl phenylacetate (729), ethyl caproate (729), ethyl isovalerate (729), α-pinene (243), α-terpineol (243) and iso valeric acid (243).