微胶囊双水相法提取罗汉松挥发油及GC-MS分析

Extraction and GC - ms analysis of volatile oil from arhat pine by microcapsule aqueous two-phase method

摘要:

本研究对罗汉松总黄酮提取工艺进行了研究，经过筛选确认了以β-环糊精-硫酸钠体系，单因素基础上通过四因素三水平响应面实验得出对罗汉松枝叶挥发油的最佳条件为萃取时间35min，β -环糊精浓度40%，硫酸钠浓度15%，萃取温度30℃，在此条件下罗汉松枝叶挥发油得率为1.372%。在质谱图中共鉴定出其中22个化合物约占色谱面积93.72 %。萜烯及其含氧衍生物18种，占总含量的73.74 %，其中萜醇类化合物4种，萜醛类化合物2种，脂肪族化合物4种，占含量的19.98 %，其中酯类化合物2种，酸类化合物2种。取得到的挥发油中主要成分为石竹烯、α -蒎烯和β -蒎烯，含量分别为17.62%、9.78%和7.47%。

Abstract:

In this study, the extraction technology of total flavonoids from Podocarpus was studied. the optimum extraction conditions of volatile oil from the leaves and branches of Podocarpus were determined as follows: extraction time 35 mi n, concentration of β -cyclodextrin 40 %, concentration of sodium sulfate 15 %, extraction temperature 30 ℃, under these conditions, the yield of volatile oil from the leaves and branches of Podocarpus was 1. 372 %. A total of 22 compounds were identified by mass spectrometry, accounting for 93.72% of the total chromatographic area. There are 18 terpenes and their oxygen-containing derivatives, accounting for 73.74 % of the total content, including 4 terpineol compounds, 2 terpinealdehyde compounds, 4 aliphatic compounds, accounting for 19.98%, including 2 ester compounds and 2 acid compounds. The main components of the volatile oil were caryophyllene, α-pinene and β-pinene, and the contents were 17.62 %, 9.78 % and 7.47 %, respectively.