海参斑软骨多糖的纯化、结构及性质分析

Purification, Structure and Properties of Polysaccharides from Lumpfish Cartilage

摘要:

以海参斑软骨为原料，采用水提醇沉得粗多糖，通过Sevag法除去蛋白，采用DEAE-52纤维素和葡聚糖G-75纯化得到组分均一的三个组份，FCPS-1，FCPS-2，FCPS-3。对三种组份进行化学成分分析并对三种组份多糖进行紫外全波长扫描，使用天青A比色法对三种多糖进行肝素效价测定，利用红外和核磁共振对FCPS-3进行结构分析，采用GPC测定FCPS-3的分子量。结果显示三种组份多糖含量均超过90%，蛋白含量低于3%，糖醛酸含量超过60%。紫外全波长扫描显示三种多糖在260nm以及280nm处均没有特征吸收峰，表示三种多糖均没有蛋白质以及核酸。红外及核磁波普显示FCPS-3为酸性多糖，且含有硫酸根，为β型吡喃糖。GPC测定FCPS-3Mw为175700Da，Z均分子量为253032Da，Mn为121885Da。

Abstract:

In this study, lumpfish cartilage as raw material,The polysacccaride of Lumpfish cartilage was extracted by water extraction, and Sevag method was used to deproteinization. The purification of polysaccharide was carried by DEAE-52 cellulose and Sephadex G-75,after dialyzed and freezed，three component polysaccharides were obtained, the three polysaccharides composition were analyzed. Full-wavelength UV was used to scan, the heparin titer of the three polysaccharides was measured by the azure A colorimetric method. The structural of FCPS-3 was analysized by infrared and nuclear magnetic resonance, the FCPS-3 molecular weight was determined by GPC. The results showed that the polysaccharide content of all three components exceeded 90%, the protein content was less than 3%, and the uronic acid content exceeded 60%.The full-wavelength UV scanning of the three polysaccharides showed that the three polysaccharides had no characteristic absorption peaks at 260 nm and 280 nm, indicating that none protein and nucleic acids were contained in three polysaccharides.The heparin titer of FCPS-3 was the highest, 1.365 U/mg, and FCPS-1 and FCPS-2 were low. Characteristic showed FCPS-3 was acid polysaccharides and contained sulfate as β-glucopyranose. the FCPS-3 Mw was 175700 Da, the Z average molecular weight was 253032 Da, and the Mn was 121885 Da.When the concentration was 5 mg/ml, DPPH•, ABTS•, OH• free radical scavenging activity of the FCPS-3 was 78.63%, 96.26%, 93.15% , respectively.