金属离子和食品添加剂对六氢β-酸/甲基-β-环糊精包合物的抑菌活性影响

Effects of Metal Ions and Food Additives on Antimicrobial Activity of Hexahydro-β-Acid/Methyl-β-Cyclodextrin Inclusion Complexes

摘要:

论文采用二倍稀释法确定了六氢β-酸环糊精包合物对7种测试菌种的最小抑菌浓度，并以活性较强的金黄色葡萄球菌和阪崎肠杆菌为指示菌，研究金属离子、食品添加剂对其抑菌活性的影响。结果表明，六氢β-酸环糊精包合物具有广谱抑菌性，除了对乙型副伤寒沙门氏菌无抑菌效果，对其他测试菌种均有良好的抑菌活性，对金黄色葡萄球菌和阪崎肠杆菌的MIC分别为16.25 μg/mL和32.5 μg/mL。金属离子Ca2 、K 、Na 、Mg2 、Zn2 对包合物的抑菌活性没有明显影响，Fe2 /3 、Cu2 对包合物的抑菌活性影响较明显。食品添加剂中NaNO2、苯甲酸钠对其抑菌活性没有明显影响，Na2SO3会略微增大对阪崎肠杆菌的抑菌活性，山梨酸钾在高浓度会降低其对阪崎肠杆菌的抑菌活性，H2O2、KMnO4在高浓度时均会明显降低其活性。

Abstract:

The minimum inhibitory concentration of HBA/M-β-CD on seven test bacteria was determined by two-fold dilution method. Take Staphylococcus aureus and enterobacter sakazakii as indicator bacteria, the effect of metal ions and food additives on antimicrobial activity was studied. The result showed that the HBA/M-β-CD inclusion complex has broad-spectrum antimicrobial activity against the tested strains, except for S. paratyphi A. And the MICs of HBA/M-β-CD against S. aureus and E. sakazakii were 16.25μg/mL and 32.5μg/mL respectively. The metal ion Ca2 , K , Na , Mg2 , Zn2 have no significant effect on their antibacterial activity of inclusion complex, conversely, Fe2 /3 , Cu2 showed significant effects on the antibacterial activity. Furthermore, for food additives, there were no significant effect on antibacterial activity of NaNO2 and sodium benzoate, and a slight effect for E. sakazakii were observed on increasing the activity of Na2SO3 and decreasing the activity of potassium sorbate in a high concentration. However, antimicrobial activity decreased with the addition of H2O2 and KMnO4 in high concentration.