高纯竹叶碳苷黄酮复合纳米生物材料的制备及其结构表征（急）

Preparation Methods and Structural Characterization of the Bio-Nanomaterial Taking High Purity C-glycoside Flavonoids of Bamboo Leaf as Leading Material

摘要:

采用离子交联法，以高纯竹叶碳苷黄酮制剂（碳苷总质量分数﹥90%，记为H-BLF）和芫根阴离子多糖（TP）为原料，通过ε-聚赖氨酸（ε-PL）的阳离子桥键合而成竹叶碳苷黄酮纳米混悬液（H-BLFnd），进一步在混悬液中添加8%（w/v）的γ-环糊精、经真空冷冻干燥得到纳米冻干粉（H-BLFnp），采用粒径分析、TEM、UV、FTIR、TG和DSC对其外观、性能、结构进行表征。结果表明： H-BLFnp外观为白色粉状，表面光洁平整，色泽均匀，平均粒径为（679±10.2）nm，分散系数为（0.349±0.004），包埋率为（92.39±0.34）%。

Abstract:

The nanodrop（H-BLFnd）was prepared by ionic cross-linking method, taking high purity C-glycoside flavonoids of bamboo leaf（H-BLF,the mass fraction of C-glycoside flavonoids was above 90 %）and turnip polysaccharide as raw materials, ε-polylysine as a cross-linking agent, then 8 % (w/v) of γ-cyclodextrin was added to H-BLFnd as protective agent, and nanoparticle (H-BLFnp) was obtained via vacuum freeze drying. The appearance, performance and structural characterization of H-BLFnp were tested through particle size analysis, TEM, UV, FTIR, TG and DSC. Results showed that H-BLFnp is a kind of white powder, smoothly and uniformly, which is of（679±10.2）nm average size, （0.349±0.004）dispersion coefficient and（92.39±0.34）% encapsulation efficiency.