响应面法优化乌腺金丝桃超声提取工艺及其抗传染性支气管炎病毒作用

Optimization of ultrasonic extraction of hypericum attenuatum by response surface methodology and its anti-infectious bronchitis virus effect

摘要:

以金丝桃素提取量为响应值，采用响应面法对乌腺金丝桃中金丝桃素影响显著的因素进行研究和优化，获得最佳提取工艺。采用细胞病变效应（CPE）法检测乌腺金丝桃提取物体外抗传染性支气管炎病毒（IBV）作用。结果建立了超声提取乌腺金丝桃中金丝桃素的工艺数学模型。超声提取金丝桃素的最佳工艺条件为提取温度40.5℃，丙酮浓度为69.5%，提取时间43.5 min，液料比为15.3，此条件下金丝桃素提取率可达到4.70mg/g，与理论值相符。主效应关系为提取时间＞丙酮浓度＞提取温度＞液料比。乌腺金丝桃提取物在细胞感染IBV前、后及同时加入，都有明显抑制CPE的作用。说明乌腺金丝桃超声提取工艺条件可靠，金丝桃素提取量远高于常规提取方法，乌腺金丝桃提取物具有很好的抗IBV作用。

Abstract:

With the amount of hypericin extracted as the response value, response surface methodology was used to study and optimize the effect of hypericin in hypericum attenuatum. The optimal extraction process was obtained. The cytopathogenic effect (CPE) method was used to detect the anti-infectious bronchitis virus (IBV) effects of hypericum attenuatum. The results showed that a mathematical model for ultrasonic extraction of hypericin from hypericum attenuatum was established，and the optimum conditions for extracting hypericin by ultrasound were as follows: extraction temperature 40.5?C, acetone concentration 69.5%, extraction time 43.5 min, ratio of liquid to material 15.3, and the extraction rate of hypericin was 4.70mg/. g, consistent with the theoretical value. The main effect relationship was extraction time > acetone concentration > extraction temperature > liquid to material ratio. The extract of hypericum attenuatum extract before, after and at the same time when cells were infected with IBV significantly inhibited the effects of CPE. It showed that the ultrasonic extraction process of hypericum attenuatum choisy was reliable, and the extractive amount of hypericin was much higher than that of routine extraction method. Hypericum attenuatum choisy had a good anti-IBV effect.