螺旋藻多糖铁配合物制备、抗氧化及淋巴细胞增殖活性分析

Preparation, antioxidant and lymphocyte proliferation activities of Spirulina Polysaccharide Iron Complex

摘要:

【目的】本研究旨在制备有机铁化合物-螺旋藻多糖铁配合物（SP-Fe），并对其结构进行分析基础上，评价其体外生物学活性。【方法】采用三氯化铁和螺旋藻多糖（SP）对SP-Fe合成进行化学改性。采用红外光谱、差示扫描量热分析、热重分析、X射线衍射和扫描电镜对其结构进行表征，分析其对抗氧化及淋巴细胞增殖活性的影响。【结果】制备SP-Fe中Fe离子含量为16.42?1.17%。采用5轴蛛网图对SP-Fe配合物的抗氧化活性进行了综合评价。SP-Fe配合物相比SP具有较高的抗氧化活性。在模拟人工胃液消化后，随着时间的延长，在人工肠液中释放出的铁含量达到83.64%；在31.25μg/mL的样品浓度下，与SP相比，SP-Fe配合物对淋巴细胞增殖提高了44.35%。【结论】SP-Fe配合物有望成为一种新型的具有良好活性的综合性铁补充剂。

Abstract:

【Objective】The aim of the present research was to prepare an organic iron compound, Spirulina polysaccharide iron complex (SP-Fe), evaluate its antioxidant activity and lymphocyte proliferation activities. 【Method】SP-Fe was prepared by chemically modification using chloride ferric and Spirulina polysaccharide (SP), which was characterized by fourier transform infrared (FTIR), differential scanning calorimetry (DSC), pyrolysis gravimetric analysis (TGA), X-ray diffraction (XRD) and scanning electron microscopy (SEM).【Results】The iron content (16.42?1.17%) of SP-Fe complex was obtained. By 5-axe cobweb charts, the results showed antioxidant activity of SP-Fe complex was higher than that of SP. The released iron content reached 83.64% in artificial intestinal juice for a prolonged time after arti-ficial gastric juice conditions. The lymphocytes proliferation of SP-Fe complex was increased by 44.35% comparing with that of SP at the sample concentration of 31.25μg/mL.【Conclusion】The SP-Fe complex had potent antioxidant activities in vitro assays and could be applied as a novel iron source in dietary supplements.