柠檬桉树脂总黄酮的提取纯化及抗氧化活性

Extraction, Purification and antioxidant activities of total flavonoid in eucalyptus citriodira resin

摘要:

采用溶剂法提取纯化柠檬桉树脂总黄酮，对粗提物(A)和纯化物进行抗氧化活性研究。用盐酸-锌粉、浓硫酸、三氯化铁、硝酸铝等方法定性鉴别树脂总黄酮，以芦丁为标准品定量分析树脂总黄酮含量(以柠檬桉树脂固体粉末的质量为基准)为4.64%。粗提物(A)经氯仿-乙酸丁酯、氯仿-二甲基甲酰胺、二甲基甲酰胺-氯仿两次超声萃取后得到纯化物B、C、D中总黄酮质量分数分别为10.39%、18.03%、44.15%。考察了A、B、C、D对2,2-二苯基-1-苦味基肼自由基(DPPH·)的清除活性、总抗氧化能力和还原能力。结果表明，A、B、C、D对DPPH·的清除活性分别为91.75%、74.22%、88.44%、91.56%；总抗氧化能力分别为19.99、1.75、4.39、3.79 μmol 芦丁当量(RE)/g；还原能力分别为1.16、0.36、0.39、0.45μmol RE/g。

Abstract:

The total flavonoids in eucalyptus citriodira were extracted and purified by solvent extraction, and the antioxidant activity and purification of crude extract(A) were studied. Total flavonoids in resin were qualitatively identified by hydrochloric acid-zinc powder, concentrated sulfuric acid, ferric chloride and aluminum nitrate. Total flavonoids content（based on the quality of eucalyptus citriodira resin solid powder） was 4.64% by quantitating analysis with rutin as standard substance. B、C、Dpurifications of different purity were obtained by chloroform-butyl acetate, chloroform-dimethyl formamide, dimethyl formamide-chloroform ultrasonic extractions. The total flavonoid mass fraction of B, C, D were 10.39%, 18.03% and 44.15%.The scavenging activities of 2, 2-diphenyl-1-bitter hydrazine (DPPH·), total antioxidant capacity and reduction capacity of A, B, C, Dwere investigated. The scavenging activities of DPPH· were 91.75%, 74.22%, 88.44%, 91.56%; Total antioxidiant activities were 19.99, 1.75, 4.39, 3.79μmol rutin equivalent(RE)/g. The reducing abilities were 1.16,0.36,0.39,0.45μmol RE/g.