藏药红景天多糖基抗菌可食膜的制备

The preparation of antibacterial and edible films based on Tibetan medicine Rhodiola rosea polysaccharides

摘要:

摘要：本研究以乙醇/硫酸铵双水相体系作为红景天多糖的活性提取溶剂，以提取的红景天多糖作为天然绿色抑菌活性因子，以甘油为增塑剂，明胶为成膜剂，氯化钙为交联剂，马铃薯淀粉为成膜糊化剂，以玻璃板作为成膜载体，采用斜面自然倾倒流延法，制备出一种新型的抗菌膜材料。以抗菌膜的抗拉强度、抑菌活性、水蒸气透过率，包肉降解特性作为膜材料的评价指标，考察成膜材质的最佳添加量。抑菌试验结果表明，制备的红景天抗菌膜材料的抑菌性仅与红景天多糖的添加量有关；当红景天多糖加入量为6 mL(质量浓度14.6%)时，抗菌膜材料对金黄色葡萄球菌和大肠杆菌的抑制率最大分别为93.04%和92.04%。抗拉强度、水蒸气透过率以及包肉降解特性实验结果表明，当多糖添加量为6 mL，甘油添加量为1.5 g、明胶添加量为1.0 g、氯化钙添加量为1.0 g时制备的抗菌膜性能最好。因此，本研究通过流延法制备的可食性膜材料，采用天然植物多糖作为抗菌因子，制作工艺简单、成本低廉、抑菌效果好、易降解、对环境无污染，是一种极具开发潜力的食品保鲜膜。

Abstract:

Abstract: In this study, the polysaccharides of Rhodiola rosea polysaccharides extracted from the aqueous two phase system of ethanol/ammonium sulphate as a active reagent be treated as the antimicrobial factor, a novel antibacterial films material would be prepared by taking the technology of natural circulation method with the glycerinum as the plasticizer, the gelatin as the filmogen, the calcium chloride as the cross-linking agent, the starch as the Pasting agent and the glass pane as the film carrier. In order to investigate the optimum additive amount of films material, the index of evaluation that it cover the strength of extension, moisture-vapor transmission, the degradation properties and antibacterial activity of films material was be established. The antimicrobial results show that the polysaccharides amounts merely contribute to antimicrobial activity, When the polysaccharide content of rhodiola rosea was 6 mL, it can obtained maximum antibacterial rate presenting to Staphylococcus aureus and Bacillus subtilis 93.04% and 92.04% respectively. The other tests results suggested that the optimum additive amount of preparing the perfect films material should be the polysaccharides 6 mL, the glycerinum 1.5 g, the gelatin 1.0 g and the calcium chloride 1.0 g. The prepared technology of antibacterial and edible films by using the natural circulation is easy to master ,to low cost, to be good antibacterial effect and to be easy degradation, which will be the enormous potential in the field of materials without the pollution to the environment.