高纯竹叶碳苷黄酮纳米材料的护肤功效（急）

Assessment on Skin Care Effect of the Composite Nanomaterial Taking High Purity C-glycoside Flavonoids of Bamboo Leaf as Leading Material (H-BLFnp)

摘要:

在采用B16黑素瘤细胞体外评价高纯竹叶碳苷黄酮复合纳米材料（H-BLFnp）美白作用的基础上，基于临床医学理论进行人体试验，应用系列皮肤测试仪器、对受试者使用试样前后的皮肤含水量、水分散失量、光泽度、明亮度、黑色素含量、皮肤弹性等生理指标进行测定，探究H-BLFnp在保湿、美白、延缓衰老方面的功效。结果显示，以β-熊果苷为阳性对照，H-BLFnp对B16细胞增殖、胞内酪氨酸酶活性和黑色素合成均有显著的抑制作用（p<0.05），当H-BLFnp质量浓度为100 μg/mL时，其抑制率分别达到45.14%、72.55%和54.01%，即其可通过降低酪氨酸酶活性、抑制黑素细胞增殖和黑色素生成达到美白功效；人体试验结果显示，H-BLFnp能显著提高皮肤的水分含量、皮肤弹性、明亮度和光泽度，降低皮肤黑色素含量和经皮水分散失。

Abstract:

H-BLFnp is a novel composite nanomaterial taking high purity c-glycoside flavonoids of bamboo leaf as leading material. Based on the whitening effect evaluated by B16 melanoma cells in vitro, its skin care function was further evaluated on the human body in this paper. The indexes of biological efficacy includes skin moisture content, percutaneous moisture loss, skin gloss, brightness, melanin content and skin elasticity were tested before and after subjects using the samples. The purpose is to explore the biological efficacy of H-BLFnp on moisturizing, whitening, anti-aging, etc. Results showed that H-BLFnp significantly inhibited B16 cell proliferation, tyrosinase activity and melanin synthesis taking β-arbutin as a positive control (p<0.05), the inhibition rates achieved 45.14%, 72.55% and 54.01% respectively when the concentration of H-BLFnp was at 100 μg/mL. The H-BLFnp may exert whitening effect through inhibiting melanocyte proliferation, tyrosinase activity and melanin synthesis. Clinic test showed that H-BLFnp can significantly increase skin moisture content, skin elasticity, brightness and gloss, and reduce skin melanin content and percutaneous moisture loss.