p（AA-co-CTS）两性型规整吸附介质的制备及其对多肽动态吸附性能

Preparation of p(AA-co-CTS) Amphoteric Continuous Adsorption Medium and Its Dynamic Adsorption Properties for Peptides

摘要:

以丙烯酸（AA）、壳聚糖（CTS）为原料单体，选用抗坏血酸（VC）作为还原剂取代传统胺类还原剂，低温冷冻聚合法1步制备了富含-COOH基团和-NH2基团的离子交换型规整分离介质p(AA-co-CTS)，聚合时间从文献报导的18h缩短至5h。聚合最优配方为：引发剂总质量分数为0.75%，其中VC与过硫酸铵(APS)的质量比为0.5，单体总质量分数为7%，CTS与AA的质量比为0.2，交联剂质量分数为1.05%，所制备的p(AA-co-CTS)规整吸附介质孔隙连续，孔隙范围为100~500μm。 p(AA-co-CTS)介质孔隙内表面丰富的-NH2、-COOH基团对多肽分子有着强吸附能力，同时以吸附传质单元数（NTU）法探究传质参数与吸附操作条件的关系，并确定最佳吸附操作条件为：吸附液流速为3~4mL?min-1，吸附温度为293.15K，进料液浓度400~500mg?L-1，最大动态吸附容量为74.5~79.4mg?g-1。所制备的两性型分离介质属于超大孔隙规整介质，可直接用于海洋渔业资源的脚料酶解液中活性多肽的吸附，操作上可取代传统吸附工艺中过滤、离心及散装树脂层析等单元操作。

Abstract:

Using acrylic acid (AA) and chitosan (CTS) as raw materials and ascorbic acid (VC) as reducing agent instead of traditional amine reductant, the contunious ion exchange adsorption medium rich in -COOH group and -NH2 group was prepared by one step copolymerization. The polymerization time was shortened from 18 hours reported in literature to 5 hours. The optimum polymerization parameters were as follows: the total mass fraction of initiator was 0.75%. The mass ratio of VC to APS was 0.5, the total mass fraction of monomer was 7%, the mass ratio of CTS to AA was 0.2, and the mass fraction of cross-linking agent was 1.05%. The pore structure of p(AA-co-CTS) adsorption medium prepared was continuous and the pore size scale was 100~500μm. The abundant groups of -NH2 and -COOH on the inner surface of p(AA-co-CTS) adsorption medium had strong adsorption capacity for polypeptide. The relationship between mass transfer parameters and adsorption operation conditions was studied by number of mass transfer units (NTU) method. The optimum adsorption operation conditions were determined as follows: the flow rate of adsorption liquid was 3~4 mL?min-1, the adsorption temperature was 293.15K, the feed concentration was 400~500 mg?L-1, and the maximum dynamic adsorption capacity was 74.5~79.4 mg?g-1. The amphoteric separation medium has continuous pores and can be directly used to adsorb active polypeptides from enzymatic hydrolysate of marine fishery resources. It can replace the traditional adsorption unit operations such as filtration, centrifugation and bulk resin chromatography.